防己水煎液总生物碱对Ⅱ型胶原诱导的大鼠关节炎及其血清中炎性因子的影响

Effcts of Total Alkaloid in Water Extraction from Stephaniae Tetrandrae Radix on Ⅱ Type Collageninduced Rat Arthritis and Inflammatory Cytokines in Serum

目的:基于防己传统临床用药多为水煎煮,对防己水煎液中总生物碱进行富集,研究其对牛Ⅱ型胶原诱导大鼠关节炎模型(collagen-induced arthritis,CIA)的影响,区别以往对其醇提物的研究,为进一步探索防己水溶性大极性生物碱的抗风湿作用提供依据。方法:采用732型阳离子树脂富集防己水煎液中总生物碱。通过足底、尾部、背部皮内注射牛Ⅱ型胶原乳剂,7 d后再加强注射1次,建立CIA大鼠模型。设正常组、模型组、防己水煎液中总生物碱低、中、高剂量组(0.162,0.324,0.486 g·kg-1)、雷公藤多苷组(1.5 mg·kg-1)和地塞米松组(10.8 mg·kg-1)分别ig给药,连续给药21 d。观察不同剂量对CIA大鼠关节炎指数、足跖肿胀度的影响,同时测定血清中白介素(IL)-1,IL-1β,IL-6和肿瘤坏死因子-α(TNF-α)水平,以及观察踝关节组织病理变化。结果:与正常组比较,模型组关节炎指数和足跖肿胀度显著升高,血清IL-1,IL-1β,IL-6和TNF-α的水平显著升高(P<0.01);与模型组比较,防己水煎液中总生物碱高、中、低剂量组能够降低关节炎指数和足跖肿胀度,明显降低血清中IL-1,IL-1β,IL-6和TNF-α的水平(P<0.01),并且减轻滑膜组织的病理损伤。结论:防己水煎液总生物碱对于类风湿关节炎有一定的治疗作用,可明显降低血清中炎性因子水平。

Objective： To study effect of total alkaloids in water extraction from Stephaniae Tetrandrae Radix S. Moore（ STRM） on the collagen-induced arthritis（ CIA） in order to further explore the anti-rheumatism effects of alkaloidthe water-soluble alkaloids with big polarity. Method： The 732 type cation resin was used to enrich total alkaloid in water extraction from STRM. Rat CIA model was established by injection of bovine Ⅱcollagen type on toes,tail and backside in rats. After 7 days same injection was performed. Alkaloids high,middle,low dose（ 0. 162,0. 324,0. 486 g·kg^-1）,Tripterygium wilfordii glycosides（ 1. 5 mg · kg^-1） and dexamethasone（ 10. 8 mg·kg^-1） were administrated for 21 days. The arthritis index（ AI） and toes swelling degree were observed,the level of interleukin-1（ IL-1）, interleukin-1β（ IL-1β）, interleukin-6（ IL-6） and tumor necrosis factor-α（ TNF-α） in serum were assayed and the ankle tissue pathological changes were observed.Result： Compared with normal group,AI in model group and toes swelling degree significantly increased（ P〈0. 01）. Compared with model group, high,middle,low dose group of total alkaloid in water extraction from STRM could improve AI and toes swelling degree,and significantly decrease the level of IL-1,IL-1β,IL-6 and TNF-α（ P〈0. 01） in serum,and reduce the pathological synovial tissue damage. Conclusion： Total alkaloid in water extraction from STRM has certain therapeutic effect on rheumatoid arthritis,and can significantly decrease the level of inflammatory factors.