金芪降糖片组分体外抗糖尿病的作用及机制

Anti-diabetic Effect and Mechanism of Components in Jinqi Jiangtang Tablet in Vitro

目的:探讨金芪降糖片各组分体外抗糖尿病的作用及机制。方法:采用DPPH自由基清除,活性氧自由基清除,AGEs形成抑制,α-糖苷酶抑制,醛糖还原酶抑制,脂肪酶抑制,脂多糖(LPS)诱发小鼠腹腔巨噬细胞活化一氧化氮(NO)释放抑制建立系统的体外抗糖尿病活性评价筛选模型,考察金芪降糖片的4个标准组分芪总黄酮、黄芪总皂苷、金银花总有机酸、黄连总生物碱对以上各模型的作用。结果:黄芪总黄酮、金银花总有机酸、黄连总生物碱具有清除DPPH自由基和活性氧自由基活性;各组分对AGEs形成无明显抑制活性;黄芪总黄酮、金银花总有机酸对糖苷酶有抑制活性;黄芪总黄酮、黄芪总皂苷、金银花总有机酸对醛糖还原酶有抑制活性;黄芪总黄酮、黄芪总皂苷、金银花总有机酸对胰脂肪酶具有抑制活性;黄芪总皂苷、黄连总生物碱对LPS诱导的小鼠腹腔巨噬细胞释放NO有抑制作用。结论:金芪降糖片具有多组分多靶点协同抗糖尿病的作用,机制涉及抑制α-糖苷酶、抑制脂肪酶、清除自由基、抑制醛糖还原酶、抑制NO释放。

Objective： To explore the anti-diabetic effect and mechanism of components in Jinqi Jiangtang tablet in vitro. Method： A systematic anti-diabetic activity evaluation screening model in vitro was established by scavenging DPPH free radicals,reactive oxygen free radicals,and inhibiting advanced glycation end products formation,alpha-glucosidase and aldose reductase,lipase,NO production fromLPS-activated mice macrophages,in order to detect the effect of four standard components—total flavonoids of Astragalus,total saponins of Astragalus,total organic acids of Honeysuckle,Berberine alkaloids in Jinqi Jiangtang tablet on the above mechanisms. Result： Total flavonoids of Astragalus,total organic acids of Honeysuckle,Berberine alkaloids showed scavenging activity on DPPH free radicals and reactive oxygen free radicals; all components showed no inhibitory activity on advanced glycation end products formation; total flavonoids of astragalus,total organic acids of Honeysuckle showed inhibitory activity of alpha-glucosidase; total flavonoids of Astragalus,total saponins of Astragalus,Total organic acids of Honeysuckle showed inhibitory activity of aldose reductase; total flavonoids of Astragalus,total saponins of Astragalus,total organic acids of Honeysuckle showed inhibitory activity of lipase;total saponins of Astragalus,Berberine alkaloids showed inhibitory effects of NO production fromLPS-induced mice macrophages. Conclusion： Jinqi Jiangtang tablet has multi-component and multi-target synergistic anti-diabetic effect and achieves the mechanisms by inhibiting alpha-glucosidase,inhibiting lipase,scavenging free radicals,inhibiting aldose reductase and inhibiting the release of NO.