摘要
为加强对虫草属核苷类化合物的研究开发,对国内外近15年虫草属核苷类成分的多种检测方法进行综述。以虫草属核苷为研究对象,对HPLC测定法、毛细管电泳法及薄层色谱扫描法等3种检测方法进行整理,并将HPLC法按不同检测器进行分类对比。HPLC法用于核苷类成分的定性、定量分析,具有灵敏度高、分离速度快和分离效率高的特点,其中,LCMS检测方法适应性广、分离能力强、检测限低,此外,还可以提供每一个组分丰富的结构信息和相对分子质量。LC-MS检测方法在核苷类成分检测中越来越广泛的应用,它不仅仅局限于虫草属核苷类成分含量测定的目的,更有利于研究人员从分子水平上开展对虫草属核苷或其他含有核苷药材的深入研究。
In order to strengthen the research and development of Cordyceps nucleosides,multiple detection methods adopted for Cordyceps nucleosides at home and abroad were reviewed. Three detection technologies used in the analysis of Cordyceps nucleoside were selected, including high performance liquid chromatography( HPLC), capillary electrophoresis( CE) and thin layer chromatography scanning( TLCs)method. According to different detectors,HPLC was also classified and compared. The HPLC method is usually applied in the qualitative and quantitative analysis of nucleosides,with a high sensitivity,quick separation and high separation efficiency. Among them,the LC-MS method features a wider adaptability,strong separation capacity and low detection limit and can provide rich structure and molecular weight information of each component. The LC-MS method has been widely used not only in the detection of Cordyceps nucleosides,but also in intensive studies on Cordyceps nucleosides or other drugs containing nucleosides at the molecular level.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第16期219-225,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81303233)
新世纪优秀人才支持计划项目(NCET-10-0944)