元江芦荟根腐病病原菌的分离及鉴定

Isolation and identification of aloe root rot pathogen

【目的】明确元江芦荟根腐病病原菌的种属地位,为其病害防治及抗病育苗奠定理论基础。【方法】采用组织分离法对芦荟根腐病病原菌进行分离、纯化及鉴定,并采用根部切伤接种法对其致病性进行测定。【结果】从芦荟病株根部共分离得到43株病原真菌,依据形态结构特征和r DNA-ITS序列测序,43株病原菌初步鉴定为7个种,归属于2个属,分别为镰刀菌属的Fusarium oxysporum、F.equiseti、F.proliferatum、F.verticillioides、F.solani和F.sambucinum等6个种(依次命名为YJLH01,YJLH02,YJLH03,YJLH04,YJLH05和YJLH06)和根霉属的Rhizopus oryzae(命名为YJLH07)。【结论】元江芦荟根腐病可能由7种病原菌共同引发,镰刀菌属可能为优势菌群。

【Objective】The present experiment was conducted to investigate taxonomical status of aloe root rot pathogens in order to provide scientific basis for controlling root rot and breeding disease-resistant seedings. 【Method】Using tissue isolation methods, the pathogens of aloe root rot was isolated, purified and identified. Then, by inoculating incised wound of aloe root with pathogen, the pathogenicity of pathogen were determined. 【Result】The results showed that43 strains of pathogenic fungi were isolated from rotting root, which were identified to be 7 species belonging to two genera based on morphological characteristics and r DNA ITS sequence. Among 7 species, six species viz., Fusarium oxysporum,F. equiseti, F. proliferatum, F. verticillioides, F. solani in turn were named as YJLH01, YJLH02, YJLH03, YJLH04,YJLH05 and YJLH06, respectively. Only one species viz. Rhizopus oryzae was named as YJLH07. 【Conclusion】The aloe root rot may be caused by these seven pathogens together, and pathogenic strains of Fusarium may be dominant microflora.