HPLC法同时测定复方双金感冒颗粒中3种活性成分的含量

Simultaneous determination of three active components in compound Shuangjin Ganmao granules by HPLC

目的：建立高效液相色谱法同时测定复方双金感冒颗粒（金银花、金莲花、板蓝根、一枝蒿、桑叶等）中荭草苷、牡荆苷、一枝蒿酮酸3种活性成分的含量。方法：采用Shim—packVP—ODS色谱柱（250mm×4．6mm，5μm），以乙腈（A）-0．4％磷酸（B）为流动相，梯度洗脱[0～38min，A—B（14：86），38—60min，A—B（40：60），60～70min，A—B（14：86）]，流速1mL·min-1，柱温30cC，检测波长340nm（检测荭草苷和牡荆苷）、242nm（检测一枝蒿酮酸）。结果：荭草苷、牡荆苷、一枝蒿酮酸质量浓度分别在16．23～97．39、5．154。68．72、9．240～55．44μg·mL-1范围内均呈良好线性关系，相关系数分别为0．9999、0．9998、0．9998；平均回收率（n：9）分别为99．45％、100．6％、99．89％，供试品溶液在24h内稳定。样品中荭草苷、牡荆苷、一枝蒿酮酸含量分别为2．703～2．712、0．338—0．344、0．736～0．742mg·g-1。结论：该方法简便、稳定、可靠可用于同时测定复方双金感冒颗粒中荭草苷、牡荆苷和一枝蒿酮酸的含量，为其质量控制提供保证。

Objective： To establish an HPLC method for simultaneous determination of orientin, vitexin and rupestonic acid in compound Shuangjin Ganmao granules（Lonicera japonica Thunb. , Trollius chinensis Bunge, Isatis indigotica Fort. ,Artemisia rupestris L. , and ,Morus alba L. etc. ）. Methods： The determination was carried out on a Shim-pack column （250 mm × 4. 6 mm, 5 μm）with the column temperature maintained at 30 ℃. The mobile phase consisted of acetonitrile （ A）-0. 4% phosphoric acid （B） solution with the gradient elution [ 0-38 min, A-B （ 14： 86）, 38--60 min,A-B（40： 60） ,60-70 min,A-B（ 14： 86） 1. The flow rate was 1 mL . min-1 and detection wavelength was set at 340 nm （ detecting orientin and vitexin） and 242 nm （ detecting rupestonic acid）. Results： The method Showed good linear relationship within the range of 16.23 - 97.39 .g .mL-1 for orientin （ r = 0. 999 9 ）, 5. 154 - 68.72 μg . mL - 1 for vitexin （ r - 0. 999 8 ） and 9. 240-55.44 g . mL - 1 for rupestonic acid （ r = 0. 999 8 ）, re- spectively;the average recoveries were 99.45% for orientin, 100.6% for vitexin and 99.89% for rupestonic acid, respectively. The sample solution was stable within 24 h. The contents of orientin, vitexin and rupestonic acid in 3 samples were 2. 703 -2. 712 mg . g-1,0. 338 -0. 344 mg . g-1and 0. 736 -0. 742 mg .g-1. Conclusion：The established method is simple, accurate and sensitive, which can be used for the determination of the three components in compound Shuangjin Ganmao granules and is helpful for quality control.