绿鳍马面鲀鱼皮蛋白抗氧化肽模拟胃肠消化制备

Antioxidant Activity and Characteristics of Simulated Gastrointestinal Digestion Hydrolysate from Filefish Navodon septentrionalis Skin Protein

为研究绿鳍马面鲀鱼皮蛋白体外消化产物的抗氧化作用及相关特征，采用二酶三阶段体外模拟胃、肠消化过程，分析产物的抗氧化作用（还原力、·OH 清除率、O2-·清除率、DPPH·清除率）和相关特征（游离氨基酸组成和含量、分子质量分布）。结果表明：绿鳍马面鲀鱼皮中富含 29.6%的抗氧化特征氨基酸；底物质量浓度在 8mg/mL 时，具有较高的抗氧化活性；抗氧化肽产生于模拟胃肠消化的十二指肠阶段，此时 DPPH·清除率为 83.29%；该阶段产物中游离氨基酸比原料中多出 7 种，各种游离氨基酸含量也明显增加；同时，产物中多肽分子量分布集中在 200～5000Da，其中主要多肽的分子量为 2152Da 和 1352Da。

To evaluate the antioxidant activity and the characteristics of hydrolysate from gastrointestinal digestion （ in vitro simulated gastric, intestinal digestion） of filefish Navodon septentrionalis skin protein, the antioxidant activity of digestive products （reducing power, OH radical-scavenging rate, O2^- radical- scavenging rate, DPPH- radical-scavenging rate） and product characteristics （amino acids composition, molecular weight distribution） were analyzed. The results showed that： the antioxidant characteristics of amino acids of the fish skin protein was rich in 29.6% ; the substrate concentration was 8 mg/mL, the hydrolysate had higher antioxidant activity; the antioxidant peptides produced mainly in the simulated duodenal digestion phase, DPPH radical-scavenging rate was 83.29% , and in this phase, seven more kinds of free amino acids in the product were found than those of raw materials. All kinds of free amino acid content were increased significantly, at the same time, molecular peptides product weight distribution concentrated in the 200 -5 000 Da, and the main peptides were at 2 152 Da and 1 352 Da which might be responsible for its antioxidant activity. The oligopeptide fraction of molecular size of 200 - 5 000 Da, revealed the highest potential capacity to scavenge free radicals and binding irons. It appears as promising ingredient to formulate functional foods with antioxidant activity.