82份水稻种质资源的稻瘟病抗性评价与抗性基因鉴定

Evaluation of blast resistance spectrum and identification of resistance genes in 82 rice germplasm resources

为了合理利用水稻抗稻瘟病种质资源,通过稻瘟病菌株接种鉴定并结合9个主效抗性基因的特异性分子标记检测,分析了82份地方稻种资源的稻瘟病抗性水平和携带的抗性基因。结果表明：在82份水稻材料中,宽抗谱和中等抗谱的材料分别有11份（13.4%）和55份（67.1%）。携带Pia、Pita-2/Pita、Pita、Piz、Piz-t和Pik-h基因的材料分别占78.0%、76.8%、54.9%、50.0%、39.0%和35.4%;各有3个材料携带Pik基因和Pib基因,未发现携带Pik-m基因的材料。大部分供试材料含有2-5个抗性基因,随着抗性基因数量的增加,供试材料的抗病性呈上升趋势。

To exploit blast resistance in rice germplasm resources, a total of 82 rice accessions were assessed for blast resistance spectrum and resistance genes distribution, using inoculation of blast isolates and screening of molecular markers tightly lined to nine major resistance genes. The results showed that 11 of 82 accessions （13.4%） had broad resistance spectrum and 55 of 82 accessions （67.1%） had medium resistance spectrum. The P/a, Pita-2/Pita, Pita, Piz, Piz-t and Pik-h genes were harbored in 78.0%, 76.8%, 54.9%, 50.0%, 39.0% and 35.4% of 82 rice accessions, respectively. The P/k and P/b genes were only harbored in three rice accessions respectively. No accession was found to contain Pik-m gene. Most of accessions contained 2-5 resistance genes. With the number of resistance genes increasing, the accession had strong resistance to rice blast.