培土生金法对脾气虚型RRTI小鼠血清IgA、肺-肠sIgA分泌的影响

Influence of Strengthening Earth to Generate Metal Therapy to Serum IgA and Lung-intestinal sIgA of RRTI Mice With Spleen-Qi Deficiency

探讨培土生金法对脾气虚型RRTI小鼠免疫机制的影响。方法:40只昆明小鼠,10只作正常对照组,余30只用苦寒泻下法建立脾气虚证RRTI小鼠模型,造模后随机分为参苓白术散组、匹多莫德组、模型对照组各10只,造模成果后开始药物干预。给药8天后检测血清Ig A,肺-肠s Ig A水平。结果:治疗后参苓白术散组可明显提高小鼠血清Ig A和呼吸道灌洗液s Ig A水平,与正常组比较和模型组比较,差异有统计学意义(P<0.05);与匹多莫德颗粒组比较,无统计学意义(P>0.05);参苓白术散组可明显提高小鼠肠道灌洗液s Ig A水平,与其余三组比较差异有统计学意义(P<0.05)。结论:培土生金法可以提高脾气虚型RRTI小鼠血清Ig A、肺-肠s Ig A分泌水平,改善脾气虚型RRTI小鼠免疫作用,疗效优于匹多莫德组。

Objective ： To explore the influence of strengthening earth to generate metal therapy on the immuno- logic mechanism of mice mice with recurrent respiratory tract infection （RRTI） due to spleen-qi deficiency. Meth- ods： There were 40 kunming mice in total. 10 mice were normal control group, the remaining 30 mice were pre- pared by purgation method with bitter cold drugs into annimal models of RRTI mice who were spleen - qi deficien- cy, they were then randomly divided into shen ling bai zhu san group, pidotimod group and model group , 10 mice in each group. Drug intervention was started after successful modeling. After 8 days of medication, the level of se- rum IgA and lung-intestinal sIgA was detected. Results ： After treatment, shen ling bai zhu san group can obvious- ly increased the level of serum IgA and sIgA in bronchoalveolar lavage fluid, compared with normal group and mod- el group, the differences were statistically significant （P 〈 0.05）, compared with pidotimod group, the difference was not statistically significant （P 〉 0.05 ） ; shen ling bai zhu san group can obviously increased the level of sIgA in intestinal fluid ,compared Conclusion： nal slzA and Strengthening with the rest of three groups, the differences were statistically significant （P 〈 0.05 ）. earth to generate metal therapy can improve the secretory level of serum IgA and lung-intesti- immunifaction in RRTI mice with spleen-qi deficiency,its curative effect was superior to pidotimod.