HPLC同时测定独一味软胶囊中的6种成分

Simultaneous determination of six kinds of components in Duyiwei soft capsules by HPLC

目的建立同时测定独一味软胶囊中山栀苷甲酯、8-O-乙酰山栀苷甲酯、芦丁、木犀草苷、毛蕊花糖苷、槲皮素等成分含量的方法。方法采用HPLC法。色谱柱为Waters Symmetry C18柱（150 mm×4.6 mm,5μm）,流动相为乙腈-水（含0.3%磷酸）,梯度洗脱,流速1 m L·min^-1,检测波长235 nm。结果山栀苷甲酯、8-O-乙酰山栀苷甲酯、芦丁、木犀草苷、毛蕊花糖苷、槲皮素的线性范围分别为0.67~10.68、1.29~20.65、1.36~21.8、0.65~10.46、0.69~10.98、0.13~2.08μg·m L^-1。平均加样回收率分别为95.1%、93.3%、98.2%、102.3%、97.6%、101.6%,RSD分别为2.6%、4.6%、3.0%、4.0%、3.5%、4.8%（n=6）。结论所用方法专属性好、精密度高,可为独一味软胶囊的质量标准研究提供参考。

OBJECTIVE To develop a method for the simultaneous determination of shanzhiside methyl ester, 8 - O - aeetyl shanzhi side methyl ester,rntin,galuteolin,verbascoside and quereetin in Duyiwei soft capsules. METHODS HPLC method was adopted. The separation was performed on Waters Symmetry Cl8 （150 mm × 4.6 mm ,5 μm）column with mobile phase consisted of aeetonitrile -water （containing 0. 3% phosphoric acid） with gradient elute. The flow rate was 1 mL·min^-1. The detection wavelength was 235 nm. RESULTS The linear ranges of shanzhiside methyl ester,8 - 0 - aeetyl shanzhiside methyl ester, rutin, galuteolin, verbaseoside and quereetin were 0.67 - 10. 68,1.29 - 20.65,1.36 - 21.8,0.65 - 10.46,0.69 - 10.98,0.13 - 2.08 μg·m L^-1, respectively. The average recoveries were 95.1% ,91.8% ,102.4% ,101.5% ,93.5% ,105.7% and the RSDs were 5.2% ,4.7% ,3.2% ,4.7% ,4.8% , 7. 1% ,respectively （n =5）. CONCLUSION The method is specific, precisive and can be applied to the analysis of Duyiwei soft capsules.