TDZ和暗培养对蝴蝶兰叶片不定芽发生的影响

Effects of TDZ and Dark Culture on Adventitious Shoots Induction from Leaf Explants of Phalaenopsis

以蝴蝶兰幼嫩花梗诱导的无菌苗叶片为外植体,以MS为基本培养基,研究不同暗培养时间和TDZ浓度对蝴蝶兰叶片诱导不定芽的影响。结果表明:暗培养和TDZ对接种的蝴蝶兰叶片的成活率均具有显著影响,暗培养60 d时,叶片的存活率在90%以上,而在同一暗培养时间条件下,叶片的存活率随着TDZ浓度的增加而上升;在TDZ浓度为3.0 mg/L,暗培养60 d时,蝴蝶兰存活叶片不定芽的诱导率最高,达到93.45%;诱导的不定芽数最多,平均每个外植体诱导的不定芽数为13.22个。综合考虑外植体的成活率、不定芽诱导率和诱导的不定芽数,蝴蝶兰叶片诱导不定芽的最佳条件为:MS+TDZ 3.0 mg/L,暗培养60 d。

Used leaves of in vitro plantlets which induced from young pedicels of Phalaenopsis as explants and the MS as basal medium, the effect of dark culture phase and TDZ on adventitious shoot induction from leaves of Phalaenopsis were investigated. The result showed that： dark culture and TDZ had a significant impact on survival rate of inoculated leaves, and the survival rate was more than 90%when the dark culture phase was 60 d, the survival rate increased with the increase of TDZ concentrations under same dark culture phase; The highest inductivity of 93.45% and maximum average number of adventitious shoots were obtained on the MS medium supplemented with 3.0 mg/L TDZ and under 60 d dark culture. Considering of survival rate, regeneration rate and adventitious shoot number, the optimum culture condition for adventitious shoots induction from leaf explants of Phalaenopsis was MS appended with 3.0 mg/L TDZ and 60 d dark culture treatment.