高压氧对兔颈内动脉和基底动脉内皮细胞凋亡影响的实验研究

Apoptotic effect of HBO exposure on cerebral vascular endothelial cells in rabbits

目的探讨高压氧(HBO)对兔颈内动脉和基底动脉内皮细胞Caspase-3和Bcl-2mRNA表达的影响。方法健康成年新西兰白兔24只,随机分为HBO组和对照组各12只。HBO组每日持续暴露于2.2ATA HBO下60min,连续3d;对照组正常饲养,不予任何处理。实时荧光定量PCR法检测Caspase-3、Bcl-2mRNA在2组颈内动脉和基底动脉内皮细胞的表达水平。结果 HBO可使颈内动脉内皮细胞Caspase-3mRNA表达明显下降[(0.038±0.006)vs.(1.000±0.225)],Bcl-2mRNA表达明显增高[(1.877±0.169)vs.(1.000±0.364)];使基底动脉内皮细胞Caspase-3 mRNA表达明显降低[(0.419±0.091)vs.(1.000±0.175)],Bcl-2mRNA的表达明显升高[(1.269±0.270)vs.(1.000±0.117)],差异均有统计学意义(P<0.01)。结论HBO对兔脑血管内皮细胞凋亡的发生有抑制作用,其机制可能与HBO对促凋亡基因Caspase-3的抑制和抗凋亡基因Bcl-2的促进作用有关。

Objective To investigate the effects of hyperbaric Oxygen （HBO） exposure on Caspase-3 and Bcl-2 mRNA ex- pressions in both internal carotid artery （ICA） and basal artery （BA） in rabbits. Methods Twenty-four healthy adult New Zealand rabbits were randomly divided into 2 groups：HBO group and the control group,with each group consisted of 12 animals. The rab- bits in the HBO group were exposed to HBO at 2.2 ATA for 60 minutes each day for 3 successive days. The rabbits in the control group were normally fed without any treatment. Real-time PCR was used to detect Caspase-3 and Bcl-2 mRNA expressions in both ICA and BA in 2 groups. Results HBO significantly decreased the Caspase-3 mRNA expression [（0. 038±0. 006）vs. （1. 000± 0. 225）] and increased the Bcl-2 mRNA expression [（1. 877±0. 169）vs. （1. 000±0. 364）]. In ICA, HBO similarly decreased the Caspase-3 mRNA expression [（0. 419±0. 091）vs. （1. 000±0. 175）] and increased the BcP2 mRNA expression [（1. 269±0. 270） vs. （1. 000±0. 117）] in BA. All the differences mentioned above were of statistical significance （P〈0.01）. Conclusion HBO ex- erts an inhibition effect on apoptosis in cerebral vascular endothelial cells. The mechanism may be related to inhibiting the expres- sion of Caspase-3 mRNA and promoting the expression of Bcl-2 mRNA.