黄芩苷对动脉粥样硬化模型大鼠NF-κBTAFI及ACE2表达水平影响

Effects of baicalin on the expression of TAFI,NF-κB,and ACE2 in the atherosclerotic rats

目的观察黄芩苷对动脉粥样硬化模型大鼠的主动脉壁细胞内核因子(NF-κB)、纤溶抑制物(TAFI)及血管紧张素转换酶2(ACE2)表达水平影响,探讨黄芩苷防治动脉粥样硬化机制。方法选取健康雄性Wistar大鼠作为实验研究对象,分为4组,每组10只,为假手术组、模型组、黄芩苷高剂量组(黄芩苷150mg·kg-1·d-1)、黄芩苷低剂量组(黄芩苷100mg·kg-1·d-1)),发色底物法测定血浆TAFI的活性,免疫组织化学法测定主动脉壁细胞内核因子NF-κB,ACE2表达水平。结果与假手术组相比,其余组大鼠TAFI、ACE2活性升高,NF-κB灰度值降低(P<0.05);黄芩苷高、低剂量组TAFI、ACE2明显低于模型组,NF-κB高于模型组(P<0.05)。结论黄芩苷可能通过下调TAFI水平,NF-κB表达,上调ACE2表达,发挥其抗动脉粥样硬化作用。

Objective To explore the effects of baicalin on the expression of TAFI,NF-~B,and ACE2 in the atherosclerotic rats and discuss the anti-atherosclerosis mechanisms of baicalin. Methods The＇subjects were healthy male Wistar rats. The rats were divided into sham operation group,model group,baicalin high dose group （150 mg · kg-1 · d-1） and baicalin low dose group （100 mg ·kg-1· d-1 ）. Plasma TAFI activity were detected with enzymatic assays. The expression of NF-kB and ACE2 were detec- ted with immunohistochemical staining. Results Compared with sham group,the rest groups of TAFI and ACE2 activity were sig- nificantly higher （P〈0.05） ,and the expression of NF-kB,had significant decreased （P〈0.05）. Compared with the model group, the level of TAFI and ACE2 in baicalin drug group was significantly lower than the model group （P〈0.05） ,and the expression of NF-kB,had significant increased. Conclusion Baicalin can reduce TAFI and ACE2 level,and upregulate the expression of NF-KB to play its role in anti atherosclerosis.