摘要
试验旨在研究猪多潜能性维持因子5(developmental piuripotency associated 5,Dppa5)基因的遗传多态性及其与母猪繁殖性状的遗传效应。以长白猪和大白猪为试验群体,采用PCR-RFLP、克隆测序等方法分析检测猪Dppa5基因的多态性并对其与母猪产仔性能进行关联分析。结果表明,猪Dppa5基因外显子上存在2个错义突变位点:g.363T>C和g.844G>T;猪Dppa5基因g.363T>C位点对初产大白猪的总产仔数有极显著影响(P<0.01),对经产大白猪、长白猪的产活仔数有显著影响(P<0.05);g.844G>T位点对经产长白猪和大白猪的总产仔数有显著影响(P<0.05)。综合结果分析,Dppa5多态位点对猪的繁殖性能存在影响,可作为潜在的遗传标记,为猪的分子育种提供参考。
The purpose of this study was to detect the genetic variation of the porcine developmen- tal piuripotency associated 5 (Dppa5) gene and its association with reproductive performance in sow. PCR-RFLP and sequencing methods were used to detect SNPs and analyze its association with reproductive performance in Large White and Landrace sows. The results showed that two missense mutations g. 363 T&C and g. 844 GZ&T were detectd in exon of porcine Dppa5. SNP g. 363 T&C affected total number born highly significantly in primiparous Large White sow (P〈 0.01) and affected litter size and number born alive significantly in Large White and Landrace sows (P〈0.05). SNP g. 844 G〉T showed a significant impact on total number born in multipa- rous Landrace and Large White sows (P 〈0.05). The results suggested that the SNPs of Dppa5 gene impacted reproductive performance of sows and could be genetic markers.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第8期2124-2130,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(30901014
31460586)
国家国际科技合作项目(2014DFA31840)