超声-低温提取藤茶中多糖和二氢杨梅素的工艺研究

Ultrasound-assisted Hypothermal Extraction of Polysaccharide and Dihydromyricetin from Ampelopsis grossedentata

研究开发了一种新颖的工艺路线实现在低温下提取藤茶中多糖和二氢杨梅素的方法。通过单因素和正交试验进行了提取工艺的优化,对提取时间、温度、超声功率和水料比四种主要影响因素设计了4因素3水平的正交试验。综合考虑正交试验的分析结果和产品性状等因素,得到了最佳提取工艺参数为水料比30︰1(m L/g),提取时间15 min,超声功率1 000 W,提取温度50℃。在最佳提取条件下,粗多糖提取率为6.74%,二氢杨梅素得率为24.37%。超声提取得到的藤茶多糖为一种糖醛酸多糖,粗产品中中性糖含量为40.11%,糖醛酸含量为41.37%。超声辅助低温提取技术得到的粗产品纯度和提取率都较高,且性状佳,可大大减少产品的后续纯化步骤。

A novel hypothermal extraction technology aided by ultrasound was developed for extracting polysaccharide and dihydromyricetin from Ampelopsis grossedentata. Through single factor and orthogonal experiments, four main extraction parameters, the extraction time, temperature, ultrasonic power and ratio of water to material were optimized by designing four factors and three levels of orthogonal experiment. Considering the orthogonal experiment results, the optimum extraction parameters were obtained： ratio of water to material 30 ： 1 （mL/g）, extraction time 15 min, ultrasonic power 1 000 W, and extraction temperature 50 ~C. Under optimum conditions, polysaccharides yield was 6.74% and dihydromyricetin yield was 24.37%. Polysaccharides obtained from ultrasonic extraction was a kind of uronic acid polysaccharide which has a neutral sugar content 40.11% and uronic acid content 41.37%. The products obtained by the hypothermal-ultrasonic extraction technology have high crude product purity and yield and better appearance. The process technology can greatly reduce the subsequent purification steps of the products.