响应面分析优化临界CO_2萃取红枣多酚及其在卷烟中的应用

Process Research of Supercritical CO_2 Extraction of Red Jujube Polyphenol with Response Surface Optimization and Its Application in Cigarette

以红枣为原料,考察超临界CO2萃取红枣多酚的效果,并对所得红枣多酚进行了卷烟加香试验。在单因素试验超临界CO2萃取时间、超临界CO2萃取压力、超临界CO2萃取温度和超临界CO2萃取次数的基础上,采用BoxBehnken试验设计,对四个因素进一步响应面分析优化,进而确定最佳工艺条件为:超临界CO2萃取时间为1.28 h,超临界CO2萃取压力为33.86 MPa,超临界CO2萃取温度为45.76℃,超临界萃取次数为2次,在此条件下得到最高的理论得率为72.75 mg/g;采用上述最优提取条件进行红枣多酚的提取试验,实际测得红枣多酚得率为72.73 mg/g,理论预测值与实测值相近。红枣多酚在卷烟加香试验表明,红枣多酚能醇和烟气,降低烟气对喉部的刺激性,降低烟气的粗糙感,改善口感。

Using red jujube as material, supercritical CO2 extraction of red jujube polyphenol was studied. Four factors, including supercritical CO2 extraction time, extraction pressure, extraction temperature, and extraction times, were determined by single factor experiment, on the basis of which, four factors were further studied with response surface optimization method by using Box-Behnken experiment design. Therefore, the optimum extraction conditions were determined. Results showed that the optimum conditions were as followings： supercritical CO2 extraction time of 1.28 h, extraction pressure of 33.86 MPa, extraction temperature of 45.76 ℃, and extraction times of 2. Under these conditions, the theoretical calculated highest product yield of red jujube polyphenol could be 72.75 mg/g, while the practical product yield of red jujube polyphenol was 72.73 mg/g, closed to the theoretical calculated result. The practical effects of perfuming indicated that red jujube polyphenol could improve tobacco aroma, smoke smoothness and after-taste of cigarette.