广西巴马人群载脂蛋白C-Ⅲ基因rs2542052位点及饮酒因素与长寿的关系

Relationship between Apolipoprotein C-Ⅲ gene rs2542052 and drinking to longevity in Bama,Guangxi

目的探讨载脂蛋白C-Ⅲ(apolipoprotein C-Ⅲ,Apo C-Ⅲ)基因rs2542052位点多态性与广西巴马长寿的相关性以及基因多态性与饮酒的交互作用对巴马长寿的影响。方法于2010年7月,抽取巴马地区152名长寿老人作为长寿组,抽取南丹地区无长寿家族史的240名健康成年人作为对照组。采用Taq Man探针实时荧光定量PCR技术对Apo C-Ⅲ基因rs2542052位点进行基因分型。结果与对照组相比,长寿组Apo C-Ⅲ基因rs2542052位点基因型和等位基因频率分布差异具有统计学意义(P<0.05);基因型与饮酒存在交互作用(P<0.05)。与CC基因型且不饮酒比较,CC基因型且饮酒不利于长寿(OR=0.142,P<0.05),而AC和AA基因型携带者不论是否饮酒均不利于长寿(OR<1,P<0.05)。结论 Apo C-Ⅲ基因rs2542052位点CC基因型携带者不饮酒更有利于长寿。

Objective To investigate the correlation between Apolipoprotein C-Ⅲ （ApoC-Ⅲ） rs2542052 polymorphisms and longevity in Bama of Guangxi,and also explore the interaction of gene polymorphisms and drinking on longevity in Bama. Methods In July 2010,a total of 152 longevity people from Bama area as longevity group and 240 healthy people without longevity family history from Nandan area as control group were selected. Genotyping of ApoC-Ⅲ （rs2542052） was performed by TaqMan real-time quantitative PCR. Results The differences in genotype and allele frequecy of ApoC-Ⅲ （rs2542052） distributions between longevity group and eontroi group were significant （P〈0.05）. Significant interaction was found between genotype and drinking （P〈0.05）. Compared with nondrinkers with CC genotype, drinkers with CC genotype was not conducive to longevity （OR=0.142, P〈0.05）, while AC and AA genotype carriers drinking alcohol or not were not conducive to longevity （OR〈1, P〈0.05）. Conclusion Nondrinkers with ApoC-Ⅲ （rs2542052） CC genotype will be beneficial to longevity.