丁苯酞预处理对脑缺血再灌注大鼠海马微血管构筑和自由基代谢的影响

Effects of 3-n-butylphthalide pretreatment on microvascular architecture and free radical metabolism in hippocampus induced by cerebral ischemia/reperfusion

目的观察局灶性脑缺血再灌注时大鼠海马微血管构筑和自由基代谢的变化,探讨丁苯酞对其影响。方法54只SD大鼠按随机数字表法分为丁苯酞预处理组、缺血再灌注组和假手术组,每组18只。线栓法制备大鼠右侧大脑中动脉栓塞模型,观察神经功能缺损评分和梗死体积;单宁酸-氯化铁(TA-Fe)媒染法显示海马微血管,定量分析微血管密度(MVD)和微血管面积密度(MVA);比色法检测海马组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果与缺血再灌注组相比,丁苯酞预处理组神经功能缺损评分和梗死体积均减少,SOD活性升高、MDA含量下降,微血管数量增多,分支吻合成网,MVD和MVA值明显升高(P<0.01)。结论丁苯酞可改善海马微血管分布,减轻自由基损伤,对局灶性脑缺血再灌注损伤有一定的预防性保护作用。

Objective To observe microvascular architecture and free radical metabolism in hippocampus after focal cerebral ischemia/reperfusion and to explore the effect of NBP （3-n-butylphthalide）. Methods Fifty-four SD rats were randomly divided into NBP pretreatment group, ischemia/reperfusion group and sham operation group （n=18 in each group）. The model of middle cerebral artery occlusion（MCAO）was established by suture method. The neurological scores were counted and the volume of infarction was measured;TA-Fe method was applied to observe the microvascular architecture of hippocampus, Mivnt image analysis system was used to analyze the microvessel density（MVD）and the microvessel area density （MVA）of hippocampus quantitatively;The activity of SOD and content of MDA were measured by colorimetric method. Results Compared to the ischemia reperfusion（IR）group, the neurological scores and the volume of infarction were decreased sharply in NBP group. What′s more, the activity of SOD, MVD and MVA were all enhanced but the content of MDA and the count of closed microvessels were both reduced（P 〈 0.01）. Conclusion NBP can improve microvascular architecture of hippocampus and reduce the free radical injury. There is a protective effect on hippocampus of rats who suffered focal cerebral ischemia reperfusion.