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流感病毒培养过程中污染预防处理研究 被引量:3

Prevention of contamination during culture of influenza viruses
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摘要 目的通过对流行性感冒病毒(流感病毒)培养过程中污染菌及其来源进行分析处理,预防和减少由于微生物的污染而影响流感病毒培养,以提高流感病毒培养阳性率,及时完成合格流感病毒毒株的报送。方法对实验室2012-2013年5次流感病毒培养受微生物污染的情况、菌种类型以及处理方法加以分析,并对可能的污染源通过抽样检查方式进行细菌培养分离,找出污染源。结果流感病毒培养过程中发生的5次实验室污染,有3次为白色假丝酵母菌污染,在一次性使用的移液管包装套上分离培养出白色假丝酵母菌;2次为缺陷短波单胞菌污染,对实验室进行空气培养时,培养出缺陷短波单胞菌,该菌为环境污染菌。结论虽然流感病毒培养时已在培养液中事先加入一定浓度的抗菌药物,但在试验操作过程中如果不能严格按照试验操作规程及实验室生物安全防护规程操作,也可造成实验室污染,导致试验失败,影响流感病毒培养结果。 OBJECTIVE To investigate the bacterial contamination and the sources during the culture of pandemic influenza viruses (influenza viruses ) so as to reduce the culture of the influenza viruses due to the microbial con‐tamination ,raise the positive rate of culture of the influenza viruses ,and submit the eligible influenza viruses strains in a timely manner .METHODS From 2012 to 2013 ,the status of 5 times of influenza viruses culture with microbial contamination in the laboratory ,species of bacteria ,and treatment methods were analyzed ,the possible contamination sources were randomly sampled for bacterial culture so as to find out the contamination sources . RESULTS Of the 5 times of contamination in the laboratory during the culture of influenza viruses ,3 times were Candida albicans contamination ,and C .albicans strains were isolated from the disposable pipette packages ;2 times were the defects shortwave Aeromonas contamination ,and the defects shortwave Aeromonas strains were cultured from the air in the laboratory , which belonged to the environmental contamination bacteria . CONCLUSION Although a certain concentration of antibiotics is added into the culture solution prior to the culture of influenza viruses ,the operations ,if not being strictly followed according to the experimental operation proce‐dures and the rules and regulations for laboratory biosafety protection ,may result in the contamination in the labo‐ratory ,lead to the failure of the experiment ,and influence the results of culture of influenza viruses .
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2015年第16期3615-3616,3628,共3页 Chinese Journal of Nosocomiology
基金 贵州省重大公共卫生基金资助项目(2100409)
关键词 流感病毒 培养 污染 预防处理 Influenza virus Culture Contamination Prevention and treatment
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  • 1齐小秋.病原生物学检验病毒[M].北京:卫生部病原生物学检验教材编写组,2009:29-31.
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