再生障碍性贫血继发流感病毒感染的危险性研究

Research on danger of aplastic anemia secondary infection with influenza virus

目的探讨流感病毒感染对再生障碍性（再障）贫血模型小鼠造血功能的影响，以期为临床再障合并流感的研究提供参考。方法采用环磷酰胺及甲苯法构建小鼠再障模型，观察再障模型小鼠与正常小鼠血红蛋白和血细胞三系的变化。判断造模效果。用微量病毒接种法判断流感病毒对正常小鼠及再障小鼠血细胞及生存的影响。结果成功建立了小鼠再障模型，再障小鼠的血红蛋白、红细胞、白细胞、血小板计数显著下降，与对照组比较差异均有统计学意义（P〈0．05）。与未接种病毒的小鼠相比，正常小鼠微量多次接种流感病毒后除发生一过性体重轻度下降外，血细胞三系均无明显变化；而再障小鼠模型接种病毒后的病死率较正常小鼠接种病毒后显著增高。结论流感病毒感染对正常小鼠的造血功能无明显影响，但可能会导致再障动物病情加重。甚至增加病死率。

Objective To investigate the influence of influenza virus infection on the hematopoietic function of aplastic anemia model mouse, and provide reference for research on clinical aplastic anemia influenza complication. Methods Small mice aplastic anemia model was set up by cyclophosphamide and toluene method. Through observing changes of hemoglobin and blood three series between model mouse and normal mouse to estimate the molding effect. Virus inoculation method was used to estimate the effects of influenza virus on the blood cell and survival of normal and aplastic anemia mouse. Results Aplastic anemia model mouse with chemical method were successfully established. Hemoglobin, red blood cell, white blood cell and blood platelet were reduced obviously, the differences compared with control group had statistical significance （P〈0.05）. In contrast with uninoculated mouse, normal mouse had no significant change in blood three series after less frequent influenza virus inoculation except for temporary body weight loss. However, the fatalily rate of aplastic anemia mouse was increased remarkably after inoculating influenza virus compared to normal mouse）. Conclusions Influenza virus has no obvious effect on normal hematopoiesis in mice, but may worsen the condition of aplastic anemia animal and even lead to illness and death.