土壤接种棉花枯萎病菌的两种方法及适宜接种量

Two methods of soil inoculation of cotton Fusarium pathogen and appropriate inoculum

本研究通过两组试验,明确了土壤接种棉花枯萎病菌的两种方法及适宜接种量。枯萎病菌的繁殖采用玉米沙粒培养基。建立苗床病圃或田间接种时,可在种沟内直接将风干的玉米沙粒培养物与土壤混匀,适宜接种量为10g/m。采用土壤拌菌法时,病原菌的风干玉米沙粒培养物的加入量以灭菌的细沙壤土的0.6%~0.8%为宜。采用上述接种方法和接种量,感病品种‘冀棉11’当年病株率可达到80%以上,病情指数可达到50以上。本文提供的接种方法和接种量可用于棉花新品种（系）、资源材料的抗病鉴定及枯萎病的相关研究。

Two sets of tests were conducted to determine two soil inoculation methods of cotton Fusarium oxyspo-rum and the appropriate quantity of inoculum.Corn and sand media was used for cultivation of F .oxysporum .To inoculate the seedling in nursery or in field,the inoculum was directly thrown into the sowing gutter and fully mixed with soils.The suitable quantity of inoculum was 10 g dried inoculum per meter.Another method is to mix dried inoculum with sterile soils fully and putting them into pots.The proportion of dried inoculum of corn and sand in-oculum in soil was 0.6% to 0.8%.By using the above two methods and quantity of inoculum,the disease incidence and disease index of ‘Jimian 11’（a susceptible variety）could reach more than 80% and 50,respectively,and the methods could be used to identify resistance of cotton varieties （lines）or materials to F.oxysporum and for related studies.