丙型肝炎患者抗黏病毒A基因的表达

Expression of Myxovirus Virus A Gene in the Patients Infected with HCV

目的:分析急性、慢性丙型肝炎患者抗黏病毒基因A(MxA)mRNA表达。方法:选择丙型肝炎病毒(HCV)感染者149例,其中,急性丙型肝炎(AHC)患者71例(AHC组),慢性丙型肝炎(CHC)患者78例(CHC组);另选体检健康人群69例为正常对照组。采用实时荧光定量PCR检测MxA mRNA表达水平及HCV RNA载量,统计分析组间各指标的差异和相关性。结果:AHC组和CHC组MxA mRNA相对表达量均明显高于正常对照组(P<0.01),且AHC组MxA mRNA的表达水平低于CHC组,差异有统计学意义(P<0.01)。AHC组MxA mRNA表达量与HCV RNA载量呈负相关(r=-0.865,P<0.001),CHC组MxA mRNA表达量与HCV RNA载量无明显相关性(r=0.174,P>0.05)。结论:急性、慢性丙型肝炎患者MxA mRNA表达水平明显增高,可以作为HCV感染的辅助诊断指标。

Objective： To explore the expression of myxovirus resistance A（MxA） in the patients infected with HCV. Method： Peripheral blood was collected from 149 patients with hepatitis C infection including 71 patients with acute hepatitis C infection（AHC） and 78 patients with chronic hepatitis C infection（CHC）. In addition, 69 healthy individuals as a control group. The expression of MxA mRNA and HCV RNA was detected by real-time quantitative PCR. Results： The expression of MxA mRNA in AHC and CHC group were significant higher than healthy group （P〈0.01）. The expression of MxA mRNA in AHC group was lower than CHC group（P〈0.01）. The expression of MxA mRNA was negative correlation with HCV RNA load in AHC group （r=-0. 865 ,P〈0.05） but no correlation with HCV RNA load in CHC group（r= 0. 174,P〈0.05）. Conclusion： The level of MxA gene expression of hepatitis C patients was significant higher than healthy person. MxA can be used as a diagnostic marker for HCV infection.