维生素D与IL-4及IL-12因子在儿童喘息性疾病中的表达及意义

Expression and significance of vitamin D and IL-4,IL-12 in children with asthmatic disease

目的探讨喘憋性肺炎和哮喘发病机理的相似性和差异性。方法选取2011年11月-2012年10月在湖南省儿童医院呼吸科住院的喘憋性肺炎患儿43例、哮喘患儿84例及儿童保健科体检的正常儿童48例为研究对象,采用酶联免疫法分别检测血清25羟维生素D(25-OH-D3)、白介素-4(IL-4)及白介素-12(IL-12)水平。结果哮喘组25-OH-D3显著低于喘憋性肺炎组及正常对照组(P<0.05);喘憋性肺炎组25-OH-D3与正常对照组比较差异无统计学意义(P>0.05);哮喘组及喘憋性肺炎组IL-4水平均显著高于正常组,而两组IL-12水平均显著低于正常组(P<0.05);哮喘组及喘憋性肺炎组之间比较,血清25-OH-D3、IL-4水平差异有统计学意义(P<0.05),IL-12水平差异无统计学意义(P>0.05)。结论哮喘和喘憋性肺炎患儿存在共同的细胞因子紊乱,推测两种疾病在发病机理上具有一定的相似性,但其病因与病理亦存在不同,有待进一步研究。

Objective To explore the similarity and difference of pathogenic mechanism between asthmatic pneumonia and asthma. Methods We selected 43 children with asthmatic Suffocating pneumonia and 84 children with asthma who were hospitalized in Department of Respiratory Diseases, the Children＇s Hospital of Hunan Province from November 2011 to October 2012, and 48 healthy children were simultaneously selected as the normal controls. ELISA was used to respectively detect the serum levels of 25 - OH- D3, interlukin - 4 （IL - 4） and interlukin - 12 （ IL - 12）. Results The level of 25 - OH - 03 of the asthma group was significantly lower than those of the asthmatic suffocating pneumonia group and the normal control group （P 〈 0.05）. No statistically significant difference was found in the level of 25 - OH - 03 between the asthmatic suffocating pneumonia group and the normal control group（P 〉 0.05）. The level of IL- 4 was significantly higher in the asthma group and the asth- matic suffocating pneumonia group than in the normal control group, while the level of IL - 12 was significantly lower in the above- mentioned two groups than in the normal control group（ P 〈 0.05）. There were statistically significant differences in the levels of 25 - OH - 03 and IL - 4 between the asthma group and the asthmatic suffocating pneumonia group （ P 〈 0.05）, but no statistically significant difference was found in the level of IL- 12 between the two groups（P 〉0.05）. Conclusions Disorders of cytokines in children with asthma are similar to these in children with asthmatic suffocating pneumonia; and hence, we speculate that there exists partial similarity in the pathogenic mechanisms of the two diseases. However, many differences in the etiology and pathology of asthma and asthmatic suffocating pneumonia still exist, and deserve further study.