摘要
目的建立齿囊耙齿菌固体发酵产物中麦角甾醇含量的测定方法。方法采用高效液相色谱方法,汉邦ODS-C18色谱分析柱(250×4.6mm,5μm),流动相为100%甲醇,流速1.0m L/min,检测波长282nm;柱温30℃。结果麦角甾醇进样量在0.20~1.40μg之间与吸收峰面积呈良好的线性关系,r=0.9999;平均加样回收率为100.07%,RSD(%)=1.91%。方法具有较高的专属性、准确性、重现性和可行性。结论该方法简便、准确、灵敏度好,为齿囊耙齿菌固体发酵产物质量评价提供了可靠依据。
Objective To establish a method for determination of ergosterol content in irpex lacteus sac fungi solid fermentation products. Methods Using high performance liquid chromatography, Hanbond ODS-C18 column chromatography (250×4.6mm, 5μm), the mobile phase of 100% methanol, flow rate was 1.0mL/ min, detection wavelength was 282nm;column temperature was 30℃. Results The injection volume of ergosterol was between 0.20-1.40μg absorption peak area, showed a good linear relationship, r=0.9999; the average recovery was 100.07%, RSD(%)=1.91%. The method had a high specificity, accuracy, reproducibility and feasibility. Conclusion The method is simple, accurate, sensitive, good, it provides a reliable basis for quality evaluation of irpex lacteus sac fungi solid fermentation product.
出处
《中国医药科学》
2015年第1期89-91,共3页
China Medicine And Pharmacy
基金
吉林省大学生创新创业项目(201410199025)