MMP-2和MMP-9在肝内胆管细胞癌组织中的表达及意义

MMP-2 and MMP-9 expressions in intrahepatic cholangiocarcinoma tissue and the significance

目的:探讨基质金属蛋白酶2(MMP-2)与基质金属蛋白酶9(MMP-9)在肝内胆管细胞癌(ICC)组织中的表达及其与ICC患者临床病理特征及预后的关系。方法:收集2011年1月—2014年12月收治的50例ICC患者的癌组织与癌旁组织手术标本,采用免疫组织化学方法及RT-PCR方法检测MMP-2和MMP-9在以上组织中的表达,分析MMP-2和MMP-9表达与ICC患者临床病理参数以及术后生存率的关系。结果:免疫组织化学结果显示,ICC组织中MMP-2和MMP-9蛋白阳性表达率分别为34.0%和32.0%,而两者在癌旁组织中无阳性表达;RT-PCR结果显示,ICC组织中MMP-2和MMP-9的相对表达均明显高于癌旁组织(均P<0.05)。MMP-2和MMP-9表达与ICC患者是否存在淋巴结转移和肿瘤分化程度有关(均P<0.05),而与患者性别、年龄和肿瘤分期无关(均P>0.05);MMP-2和MMP-9阳性患者生存率明显低于各自阴性者,而两者均阳性患者的生存率最低(均P<0.05)。结论:ICC组织中MMP-2和MMP-9表达明显上调,且两者的表达与ICC患者恶性临床病理特征及不良预后密切相关。

Objective： To investigate the expression of matrix metalloproteinase 2 （MMP-2） and 9 （MMP-9） in intrahepatic cholangiocarcinoma （ICC） and their relations with the clinicopathologic features and prognosis of ICC patients. Methods： The surgical specimens including tumor and tumor-adjacent tissue from 50 ICC patients treated between January 2011 and December 2014 were collected. The MMP-2 and MMP-9 expressions in these tissues were detected by immunohistochemical staining and RT-PCR method, and the relations of MMP-2 and MMP-9 expressions with clinicopathologic variables and postoperative survival of ICC patients were analyzed. Results： Results ofimmunohistochemical staining showed that the positive expression rate of MMP-2 and MMP-9 in ICC tissue was 34.0% and 32.0% respectively, but neither was positive in tumor-adjacent tissue; results of RT- PCR showed that both relative expression levels of MMP-2 and MMP-9 in ICC tissue were significantly higher than those in tumor-adjacent tissue （both P〈0.05）. Both MMP-2 and MMP-9 expressions were significantly associated with the presence or absence of lymph node metastasis and degree of tumor differentiation of ICC patients （all P〈0.05）, but were irrelevant to the gender, age and tumor stage of ICC patients （all P〉0.05）; the survival rate in patients with positive MMP-2 or MMP-9 expression was significantly lower than that in patients with negative MMP-2 or MMP-9 expression, and those with both MMP-2 and MMP-9 positive expressions had lowest survival rate （all P〈0.05）. Conclusion： MMP-2 and MMP-9 expressions are increased in ICC tissue, and both expressions are closely related to the malignant clinicopathologic profiles and poor prognosis of ICC patients.