D-IBS与FD重叠征大鼠精神状态、胃肠激素水平改变

Mental state,gastrointestinal hormone level changes of D-IBS and FD overlap syndrome rat model

目的探讨功能性消化不良(functional dyspepsia,FD)与腹泻型肠易激综合(diarrhea irritable bowel syndrome,D-IBS)重叠征动物模型的精神状况及其与脑肠肽、细胞因子水平差异的相关性。方法将50只健康Wistar雄性大鼠按随机数字表法分组,观察大鼠正常组及造模组行为变化、血浆中血管活性肠肽(VIP)、生长抑素(SS)、5-羟色胺(5-HT)、内毒素(ET)、白细胞介素-10(IL-10)和结肠组织中白细胞介素-2(IL-2)的水平。结果与正常组比较,D-IBS与FD重叠征大鼠糖水消耗百分比显著降低(P<0.01),平均活动速度显著降低(P<0.05),血浆SS显著降低(P<0.01),血浆VIP水平显著升高(P<0.01);血浆5-HT及IL-10变化差异无统计学意义,组织中IL-2表达阳性细胞比差异无统计学意义;与单纯D-IBS组比较,FD+D-IBS组(B组)血浆ET水平显著升高(P<0.01)。结论 D-IBS与FD重叠征大鼠模型存在精神异常;其症状重叠可能与血浆VIP、ET水平升高及SS降低有关,与精神异常、血浆IL-10水平及结肠组织IL-2免疫反应细胞无明显相关性。

Objective To investigate the psychological status, physical and biochemical indicators, and behavioral changes in the model of diarrhea type irritable bowel syndrome （D-IBS） and functional dyspepsia （FD） overlap syn- drome. Methods Fifty healthy male Wistar rats were randomly divided into five groups. Behavioral changes were ob- served in normal group and model groups. Levels of vasoactine intrestinal peptide （ VIP）, somatostatin （ SS）, 5-hydroxy tryptamine （5-HT）, endotoxin （ET） and interleukin-10 （IL-10）, as well as interleukin-2 （IL-2） levels in the colon were examined. Results Compared with normal group, the D-IBS and FD overlap syndrome rats had lower sugar con- sumption percentage （ P 〈 0.01 ）, lower average activity speed in the open field （ P 〈 0.05 ）, lower SS levels （ P 〈 0.01 ） , but higher VIP levels （P 〈 0.01 ）. Compared with D-IBS group, endothelin levels were significantly increased in FD ＋ D-IBS group （P 〈 0.01 ）. Conclusion Mental disorders exist in D-IBS and FD overlap syndrome models of rats, which are related to the increase of VIP and ET levels and the decrease of SS.