溃疡性结肠炎外周血CD3^+、CD4^+、CD8^+ T细胞的活化及其与炎症标志物的关系

Activation of CD3^+,CD4^+ and CD8^+T cells in peripheral blood of ulcerative colitis and the relationship with inflammatory markers

目的研究溃疡性结肠炎(ulcerative colitis,UC)患者外周血CD3+、CD4+、CD8+T细胞变化与炎症相关指标的关系。方法收集25例UC患者和7名健康对照者外周血,用流式细胞仪检测CD3+、CD4+、CD8+T细胞的比例及表型,并与炎症相关指标(白细胞计数、血小板计数、血沉、CRP、白蛋白)进行相关性分析。结果 UC及活动期UC患者外周血CD3+、CD4+和CD8+T细胞的比例明显高于对照组,差异有统计学意义(P<0.05);缓解期UC虽略高于对照组,但仅CD8+差异有统计学意义(P<0.05)。UC患者白细胞计数、血小板计数、CRP和白蛋白明显高于对照组(P<0.05);UC活动期的血小板计数、CRP、血沉和白蛋白水平明显高于健康对照组(P<0.05)。CD3+、CD8+T细胞比例与CRP、血沉呈明显正相关,CD4+比例与CRP呈明显正相关(P<0.05)。结论UC患者外周血T细胞活化,并与炎症相关指标CRP和血沉呈正相关。

Objective To evaluate the relationship of inflammation-related markers with circulating T-cell activation in human subjects with ulcerative colitis （UC）. Methods Twenty-five patients with UC and 7 healthy controls were collected. T-cell proportion and phenotype were assessed in whole blood samples by flow cytometry. Correlation of T-cell populations with inflammatory markers （including white blood cell count, platelet count, erythrocyte sedimentation rate （ESR） , CRP and albumin） in patients with UC were analyzed. Results The proportions of circulating CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphoeytes in cycle were increased in patients with UC and active UC compared with controls （P 〈 0.05 ）. These proportions in remission UC were slightly higher than controls, but only CD8 ＋ T lymphocytes had signifi- cant difference （P 〈0.05）. The white blood cell count, platelet count, CRP and albumin levels of UC patients were significantly higher in remission UC than controls （P 〈 0.05）. The platelet count, CRP, ESR and albumin levels of ac- tive UC patients were significantly higher than controls （P 〈 0.05 ）. The proportions of CD3 ＋ and CD8 ＋ T cell were positively correlated with CRP and ESR, respectively, and CD4＋was positively correlated with CRP （P 〈 0.05）. Con- clusion Peripheral CD3 ＋ , CD4 ＋ and CD8＋ T cells are activated in UC and are associated with inflammation-related markers CRP and ESR.