眼镜蛇毒溶解因子对人鼻咽癌细胞株的毒性及其与抗癌药的协同作用

Cytotoxicity of cobra venom lytic factors on cultural human nasopha ryngeal tumor cell line and its synergism with antitumor drugs

目的 :探讨纯化的眼镜蛇毒因子 (CVF) ,CVF和单抗交联物 (BAC5 -CVF)和直接溶解因子 (DLF)对人鼻咽癌细胞株的毒性及DLF和抗癌药的相互作用。方法 :用MTT法或台盼蓝拒染法了解合并用药的细胞毒作用。结果 :CVF本身对CNE2细胞并无细胞毒性 ;BAC5 -CVF对CNE2细胞有剂量依赖性杀伤作用 ,其IC5 0为 3 0 7× 10 -7mol/L ;引起 2 2 0 3 %抑制效应的DLE与长春碱 (VLB)、甲氨蝶呤 (MTX)、阿霉素 (ADM)、氟尿嘧啶 ( 5 -FU)和平阳霉素 (BLM)有协同作用 ,但能拮抗氮芥 (HN2 )作用。结论 :CVF不引起CNE2细胞毒性 ,但BAC5 -CVF和DLE对CNE2细胞有毒性作用 ;除能拮抗氮芥外 。

Objective:To investigate the cytotoxicity of cobra venom factor(CVF),immunoconjugate(BAC5-CVF),and direct lytic fator(DLF)on cultural human nasopharyngeal tumor cell line(CNE2)and the synergism of DLF on antitumor drug cytotoxicity. Methods:The cytotoxicity was detected using MTT method or typanblue exclusion test,the enhancement was confirmed with the combination of DLF in the level inducing 20%growth inhibition and antitumor drugs in the level inducing 50%growth inhibition of CNE2 cells. Results:No any cytoxicity of CVF itself on CNE2 cells was found,but BAC5-CVF can induce cytotoxicity on the same cell line in dose dependent manner,the IC50 is 3 07×10 -7 mol/L.DLF can enhance cytotoxicity of conventional antitumor drugs including VLB,MTX,ADM,5-FU,and BLM,but antagonize that of HN2. Conclusions:Both BAC5-CVF and DLF have cytotoxicity on CNE2 cells,and DLF can enhance cytotoxicity of the conventional antitumor drugs on cultural CNE2 cells except NH2.