养猪废水中重金属-抗生素双抗菌的筛选

Screening of Dual Resistant Strains of Antibiotic and Heavy Metal from Swine Farm Effluent

为了筛选重金属-抗生素双抗菌,从海口市郊某养猪场养猪废水中分离优势菌,将优势菌株涂布在二元交叉抗性试验培养基上,观察菌株生长情况.结果显示,从养猪废水中分离出的优势菌共有10株,其中7#菌株能在11种交叉抗性板上生长,是交叉抗性最多的菌.其他菌株对不同交叉培养基有不同的抗性结果.结果表明,筛选出的优势菌中,大多数对养猪业最常用的饲料添加剂盐酸四环素、磺胺二甲嘧啶、杆菌肽锌、Na As O2、Cu Cl2、Zn SO·7H4 2O产生抗性;这是微生物长期处于抗生素和重金属微污染环境下产生适应和微进化的结果.

In order to screen cross-resistant strains of heavy metals and antibiotics （H-B）, dominant strains were isolated from swine farm effluent collected in the suburb of Haikou. 10 strains with dual resistance of antibiotic and heavy metal were screened from the colonies growing on cross H-B agar medium. Among them, strain 7# is the most resistant one, it could grow on 11 H-B media, and others have different performances on different H-B media. The results showed that most of dominant bacteria are resistant to tetracycline hydrochloride, sulfadimidine, bacitracin zinc, NaAsO2 resistant, CuCh, ZnSO4. 7H20, which are the most regularly used in swine feed supplements. It reflects that the adaptation and microevolution have been taking place in the bacteria under long-term coselective pressure of heavy metals and antibiotics.