HPLC法测定山术运肠丸中阿魏酸的含量

Determination of ferulic acid in Shanshu Yunchang Pills by HPLC

目的建立高效液相色谱法测定山术运肠丸中阿魏酸的方法.方法采用高效液相色谱法,以SunfireC18（4.6mm×250mm,5μm）为色谱柱,流速为1mL·min^-1,乙腈-0.1%磷酸溶液（18∶82）作为流动相,检测波长316nm.结果阿魏酸在0.118～1.422μg范围内呈良好线性关系（r=0.9999）,回收率为98.46%,RSD=1.21%.结论该方法简便,快速准确,可以作为山术运肠丸中阿魏酸的含量测定.

Objective To establish an HPLC method for the determination of ferulic acid in Shanshu Yunchang Pills.Methods The analysis was perfermed on Sunfire C18colunm( 4. 6 mm × 150 mm,5 μm) with a rate of 1 m L·min- 1. The mobile phase was composed of acetontrile and 0. 1% phosphoric acid solution( 18∶82). The detection wavelention was 316 nm. Results The linear range of ferulic acid was 0. 118 ~ 1. 422 μg( r = 0. 999 9) and the average recovery of ferulic acid was 98. 46%( RSD = 1. 21%). Conclusion The method was simple,fast and accurate. It was suitable for the determination of ferulic acid in Shanshu Yunchang Pills.