高效液相色谱法测定菌群降解纤维素产物中的糖、有机酸和醇

Determination of sugars,organic acids and alcohols in microbial consortium fermentation broth from cellulose using high performance liquid chromatography

采用硫酸除钙和调节p H的样品预处理方法,利用高效液相色谱对菌群降解纤维素发酵液中的糖、有机酸和醇3类物质进行分析,待测组分与培养基成分能够得到有效分离。从菌群降解纤维素发酵液中检出纤维二糖、葡萄糖、乙醇、丁醇、甘油、乙酸与丁酸并进行了定量分析,7种组分的检出限范围为0.10～2.00 mg/L,线性相关系数均大于0.999 6,线性范围为0.020～1.000 g/L,回收率为85.41%～115.60%,相对标准偏差（RSD）为0.22%～4.62%（n=6）。该方法准确可靠,可实现对菌群降解纤维素发酵液中糖、醇和有机酸的同时准确测定。

A high performance nation of metabolites （ sugars, liquid chromatographic method organic acids and alcohols ） in was established for the determi- microbial consortium fermenta- tion broth from cellulose. Sulfate was first added in the samples to precipitate calcium ions in microbial consortium culture medium and lower the pH of the solution to avoid the dissociation of organic acids, then the filtrates were effectively separated using high performance liquid chromatography. Cellobiose, glucose, ethanol, butanol, glycerol, acetic acid and butyric acid were quantitatively analyzed. The detection limits were in the range of 0. 10-2.00 mg/L. The linear correlation coefficients were greater than 0.999 6 in the range of 0. 020 to 1.000 g/L. The recoveries were in the range of 85.41%- 115.60% with the relative standard deviations of 0.22% -4.62% （n =6）. This method is accurate for the quantitative analysis of the alcohols, organic acids and saccharides in microbial consortium fermentation broth from cellulose.