肿瘤坏死因子Ⅱ型受体基因多态性与特发性间质性肺炎的相关性研究

Idiopathic Interstitial Pneumonia in relation to the Gene Polymorphisms of Tumor Necrosis Factor Receptor Ⅱ

目的研究肿瘤坏死因子Ⅱ型受体(TNFRⅡ)196位点基因多态性与特发性间质性肺炎(IIP)的关联。方法累积收集100例特发性间质性肺炎(IIP)患者,包括特发性肺纤维化(IPF)83例,非IPF-IIP 17例。选取100例年龄、性别、民族、吸烟情况匹配的同地区的健康对照者。应用多聚酶链反应-限制性片段长度多态性技术(PCRRFLP)分别检测入选病例及对照者的肿瘤坏死因子Ⅱ型受体(TNFRⅡ)196位点的基因多态性,并统计分析各基因分布频率与发生IIP、IPF的相关性。结果 IIP组与对照组、IPF组与对照组之间的TNFRⅡ196位点的基因型频率和等位基因频率分布在统计学上均无显著性差异(P>0.05)。IIP、IPF组和对照组比较,突变基因型196R/R+196M/R的OR值分别为1和1.057,95%CI为0.552-1.812和0.550-2.030。TNFRⅡ196位点的突变基因型与IIP和IPF的发生无显著相关性。结论中国汉族人群TNFRⅡ196位点基因多态性可能与IIP、IPF的发生无关联。

Objective To observe the relationship between the gene polymorphisms of tumor necrosis factor receptor II（TNFRⅡ）196 locus and idiopathic interstitial pneumonia （IIP）.Methods 100 consecutive patients with idiopathic interstitial pneumonia were subgrouped idiopathic pulmonary fibrosis（IPF,n = 83）and non-IPF（n = 17）.100 healthy controls matched in age,sex,race and smoke state came from the identical area were selected.Polymerase chain reaction （PCR）and restriction fragment lengthpolymorphism （RFLP）were performed to analyze tumor necrosis factor receptor II 196 locus gene polymorphisms in the people.Polymorphisms affecting IIP and IPF incidence were calculated by SPSS.Results There were no significant differences in the genotype distribution and the allele gene distribution of TN-FRⅡ196 locus between IIP and IPF patients and controls （P 〉0.05）.The OR of 196R/R＋196M/R mutational geno-type for IIP were 1 and 1.057（95%CI：0.552-1.812 and0.550-2.030）,respectively.There was no significant associa-tions between TNFRⅡ196 locus genotype and the predisposition to IIP and IPF.Conclusion It is concluded that TN-FRⅡ196 locus gene polymorphism may have no effect on the predisposition to IIP and IPF.