摘要
以发光二极管为激发光源,以多模光纤代替透镜组作为激发光路,利用毛细管作为检测池,研制了一台小型、低功耗的荧光检测器。采用正交型光路设计,借助高精度机械加工的自校准平台,使光纤-毛细管-荧光收集透镜实现自校准。采用流动注射模式,以荧光素钠为标准样品对该检测器性能进行了测试,最低检测限为6.75×10-8 mol·L-1（S/N=3）,在1.35×10-7～43.2×10-7 mol·L-1范围内线性关系良好（R2=0.9992）,稳定性良好（RSD〈1%）。结合高效液相色谱系统,分离了异硫氰酸荧光素标记的4种氨基酸。
A small and low power efficient fluorescence detector was developed using light-emitting diode as excitation light source, multimode optical fiber as excitation light and capillary as testing pool. Based on orthog- onal optical Arrongement,a homemade high precision machined platform was utilized in the self-alignment and self-calibration of optical fiber,capillary,and fluorescent collection lens. To evaluate the performance of the de- tector,a flow injection moldel was applied using sodium fluorescence as standard sample. Results showed that, the limit of detection（LOD） was 6.75×10 mol·L-1（S/N=3）.linear relationship was good in the range of 1.35×10 7 ～43.2× 107 mol·L-1（R2=0.9992） with good stabitity（RSD〈1%）.Four kinds of amino acids labeled by fluorcescein isothiocyanale（FITC） were separaled by the detector via HPLC method.
出处
《化学与生物工程》
CAS
2015年第8期48-52,共5页
Chemistry & Bioengineering
关键词
发光二极管
光纤
荧光检测器
荧光素钠
light-emitting diode
optieal fiber
fluorescence detector
sodium fluorescence
