电子烟烟气捕集液对CHO细胞相对增殖率的影响

Effect of Electronic Cigarette Smoke in Cell Culture Medium on Relative Growth Rate of Cell CHO

参照加拿大Labstat的电子烟抽吸模式,抽吸100口电子烟,用细胞培养基捕集电子烟烟气,通过MTT法研究电子烟烟气对CHO细胞相对增殖率的影响。结果表明:在该抽吸模式下,抽吸100口电子烟产生的烟气捕集液与CHO细胞相对增殖率之间存在浓度梯度效应;抽吸一支3R4F产生的烟气捕集液对CHO细胞相对增殖率的影响明显高于抽吸100口电子烟所产生烟气的影响,在100%烟气浓度下,电子烟烟气捕集液对CHO细胞的相对增殖率是3R4F的10倍以上。

Based on Canada Labstat electronic cigarette suction mode, every 100 puffs of electronic cigarette smoke were trapped into cell culture medium, effect of electronic cigarette smoke on relative growth rate（RGR） of cell CHO was studied via MTT assay. Results showed that,in this suction mode,there were concentration gradient effect between RGR of cell CHO and 100 puffs of electronic cigarette smoke in cell culture medium,one 3R4F had higher effect than 100 puffs electronic cigarette,the RGR of 100 puffs of electronic cigarette smoke in cell culture medium was more than 10 times of one 3R4F at concentration of 100%.