麻杏二陈汤煮散与饮片止咳祛痰作用的比较

Comparion the Effects of Maxingerchentang(麻杏二陈汤) Zhusan(煮散) and Chinese Medicinal Pieces on Eliminating Phlegm and Stopping Cough

目的:通过动物实验,比较麻杏二陈汤煮散与饮片的止咳祛痰作用。方法:采用小鼠氨水引咳方法,将小鼠随机分成6组,每组12只,每天灌胃给药1次,连续灌胃给药7 d,空白组及阳性对照组分别给予生理盐水和复方甘草合剂,各组于最后一次给药1 h后用25%氨水按预定的时间喷雾刺激,观察小鼠1 min内出现的咳嗽次数,计算R值。另外,采用气管段酚红法观察祛痰作用,按上述方法分组及给药,每组10只小鼠。末次给药后45 min,腹腔注射酚红溶液,测定吸光度,计算酚红含量。结果:麻杏二陈汤煮散和饮片高剂量组均有显著止咳作用,结果显示R值均大于130%,两者的低剂量组作用相对较弱;与空白对照组比较,麻杏二陈汤煮散高、低剂量组、饮片高剂量组能显著增加小鼠气管酚红排泌量,差异有统计学意义(P<0.05)。煮散与饮片同剂量组比较,差异无统计学意义(P>0.05)。结论:麻杏二陈汤具有较好的止咳祛痰作用,煮散高剂量组和低剂量组比饮片相应组的剂量低一倍,但作用相当。

Objective： To compare the effects of Maxingerchentang zhusan （MXZ） and Chinese medicinal pieces （CMP） on eliminating phlegm and stopping cough by animal experiments. Methods： The cough model of mice was applied, which were induced by ammonia and the mice were randomly divided into six groups, 12 in each group. Blank group was given normal saline, and positive control group was given brown mixture, and other groups were administrated with drugs for 7 days, and once a day. And all the mice were stimulated by atomizing with 25% ammonia. After 1 h of the final administrated, and then delitescence and cough time were recorded during one minute, and the value of R was calculated. In addition, the effect of eliminating phlegm was examined by the method of phenol red; 45 min after last administration, the absorbance was detected, and the content of phenol red was calculated. Result： The MXZ and CMP high-dose could reduced cough time during one minute; the results showed that the value of R were greater than 130%, but the MXZ and CMP low-dose group have the role of weak. Compared with the blank group, the content of phenol red were obviously increased by the MXZ and CMP high-dose and the MXZ low-dose group, and the difference was statistically significant（P〈0.05）. But the difference showed no statistical significance between the same dose group of MXZ and CMP （P〉0.05）. Conclusion： The MXZ and CMP have favourable effect on cough-relieving and sputum-removing in mice. The effect is same when the dose of CMP twice as much as the MXZ.