延迟性肌肉酸痛动物模型的建立及组织形态学观察(英文)

Establishing an animal model of delayed onset muscle soreness and its histomorphologic observation

背景:延迟性肌肉酸痛和骨骼肌微损伤有密切关系,但对于骨骼肌微损伤的确切机制还不太清楚。目的:通过离心运动建立骨骼肌微损伤模型,通过其组织形态学和超微结构变化。方法:将40只雄性SD大鼠随机等分为安静对照组、运动后即刻组、运动后24 h组、运动后48 h组和运动后72 h组。后4组大鼠进行一次间歇性下坡跑运动,速度为16 m/min,坡度为-16°,5 min运动,2 min休息,共120 min。运动后即刻组、运动后24 h组、运动后48 h组和运动后72 h组大鼠分别于运动后即刻、24,48,72 h进行观察。结果与结论:离心运动后,大鼠骨骼肌组织形态结构和超微结构都发生了程度不同的变化,骨骼肌组织中结蛋白和波形蛋白出现不同程度的抗结蛋白抗体染色脱染。表明一次性离心运动能引起延迟性骨骼肌微损伤。

BACKGROUND：Delayed onset muscle soreness is closely related to skeletal muscle micro-injury, but the exact mechanism of skeletal muscle micro-injury is not yet clear. OBJECTIVE：To observe the histomorphological and ultrastructure changes of skeletal muscle micro-injury models induced by eccentric exercise. METHODS： Forty male Sprague-Dawley rats were randomly divided into quiet control group, immediately after exercise group, post-exercise 24 hours group, post-exercise 48 hours group and post-exercise 72 hours group. In the latter four groups, the rats were subjected to intermittent running on the-16° slope at a speed of 16 m/min： 5 minutes movement, 2 minutes rest and totaly 120 minutes. Rats in the latter four groups were observed immediately, at 24, 48, 72 hours after exercise. RESULTS AND CONCLUSION：After eccentric exercise, the morphology and ultrastructure of rat’s skeletal muscle were both changed to different extents, and Desmin and Vimentin were dyed off for anti-desmin antibody staining at varying degrees. It indicates that one-time eccentric exercise can cause delayed skeletal muscle micro-injury.