高效液相色谱测定3种活性成分的含量

Quantitative Determination of Three Active Constituents by High Performance Liquid Chromatography

目的采用高效液相色谱测定小儿肺热咳喘口服液中盐酸麻黄碱、盐酸伪麻黄碱和苦杏仁苷含量。方法采用RP-HPLC法测定,选择流动相为:甲醇-乙腈-水(9∶9∶82,加0.1%三乙胺),检测波长为210 nm。结果此方法精密度高,重现性好,盐酸麻黄碱平均回收率为99.42%,RSD(%)=0.38,盐酸伪麻黄碱平均回收率为99.23%,RSD(%)=0.35,苦杏仁苷平均回收率为99.32%,RSD(%)=0.43。结论此方法简便、灵敏、准确,可用于盐酸麻黄碱、盐酸伪麻黄碱和苦杏仁苷的含量测定。

Objective To determine the content of ephedrine hydrochloride, pseudoephedrine hydrochloride and bitter apricot seed in Xiao＇er Feire Keehuan Oral Solution by high performance liquid chromatography. Methods ARP - HPLC method was estab- lished, with emethanol - acetonitrile - water （v ： v ： v = 9 ： 9 ： 82, adding 1% triethylamine） used as the mobile phase, and 210 nm was the detecting wave length. Results The method has higher precision and repeatability. The recovery rate of Ephedrine Hydrochlo- ride was99.42% , RSD （%） =0.38, the rate recovery of pseudoephedrine Hydrochloride was 99.23% , RSD （%） =0.35, and the recovery rate of Bitter Apricot Seed was 99. 32%, RSD （ % ） = 0.43. Conclusion With the advantages of simplicity, sen- sitiveness and accuracy, the method can be used to determine the content of ephedrine hydroehloride, pseudoephedrine hydrochloride and bitter apricot seed.