胶质瘤中SOX2和Cyclin D1的表达及意义

Expressions of SOX2 Cyclin D1 in Glioma and their clinical significance

目的探讨SOX2和细胞周期素D1(Cyclin D1)在人脑胶质瘤中的表达及意义。方法收集58例胶质瘤和10例正常脑组织标本,应用实时荧光定量PCR技术检测胶质瘤组织及其正常脑组织中SOX2和Cyclin D1基因的表达水平。结果实时荧光定量PCR结果显示胶质瘤组织中SOX2和Cyclin D1的mRNA水平显著高于正常脑组织,且SOX2和Cyclin D1在高级别病理分期胶质瘤中的表达水平显著高于低级别组(P=0.002,P=0.0064)。胶质瘤组织中SOX2和Cyclin D1的表达水平具有相关性(P=0.01)。结论 SOX2和Cyclin D1在胶质瘤组织中异常高表达,且二者表达水平显著相关,提示SOX2和Cyclin D1可能协同参与了胶质瘤的发生及发展过程。

Objective To investigate the expression and clinical significance of SOX2 and CyclinD1 in human glioma. Methods 58 cases of glioma tissues and 10 cases of normal brain tissue specimens were enrolled in this study. Real - time RT - PCR assay was used to detect the mRNA level of SOX2 and CyclinD1 expression in glioma tissues and normal brain tissues. Results Real - time RT - PCR results showed that the mRNA levels of both SOX2 and CyclinD1 in glioma tissues were significantly higher than those in the normal brain tissue （ P = 0. 002 and P = 0. 0064, respectively）. In addition, the SOX2 and CyclinD1 expressions in glioma tissues with higher pathological grade were also higher than that in the lower grade tissues, and there was positive relationship between the ex- pressions of SOX2 and CyclinD1 in glioma tissues. Conclusion he expression of SOX2 and CyclinD1 was abnormally increased in dif- ferent pathological grade glioma tissues in a manner with a positive relation, suggested that both SOX2 and CyclinD1 might be involved in the occurrence and development of glioma.