市售鲜畜肉中沙门菌的血清型及耐药性研究

Studies on the serotypes and drug resistance of Salmonella isolated from retail fresh meat of livestock

为了研究市售新鲜畜肉(猪肉、牛肉)中污染的沙门菌血清型及其耐药性,试验以2012—2014年南宁市售384份新鲜猪肉、牛肉分离得到的92株沙门菌疑似菌株为研究对象,试验采用GB4789.4—2010血清学鉴定的方法以及Kirby-Bauer法分别检测分离株的血清型及耐药性。结果表明:从分离株中共鉴定出21种血清型,主要血清型为德比沙门菌(51.1%)以及伦敦沙门菌(13.0%);分离株对四环素的耐药率最高(70.7%),其次为氯霉素、甲氧嘧啶、复方新诺明及氨苄西林,除11株对20种测试药物全部敏感外,其余81株均表现不同程度的耐药性,其中三重及以上的耐药菌株占56.5%,分离株最多可耐10种抗生素。说明南宁市售新鲜猪肉、牛肉中污染的沙门菌血清型复杂,多重耐药性问题比较严重。

To investigate the epidemic serotypes and drug resistance of Salmonella isolated from retail fresh meat samples including beef and pork in Nanning, 92 suspected Salmonella isolates were used as research object, which were isolated and obtained from 384 copies of retail fresh pork and beef in Nanning during 2012 to 2014. A serological identification method from GB 4789.4--2010 and Kirby - Bauer method was used to detect the serotypes and drug resistance of the isolates, respectively. The results showed that a total of twenty - one kinds of seretypes were iden- tified from the isolates. Thereinto, Salmonella Derby （51.1% ） was the dominant seretype, followed by Salmonella London （ 13.0% ）. The iso- lates showed the highest resistance to tetracycline （70.7 % ） followed by chloramphenicol, trimethoprim, cotrimoxazole, and ampicillin. 11 iso- lates were sensitive to all of the 20 drugs, the remaining 81 isolates showed different degrees of resistance. Thereinto, 56.5 % isolates showed resistance to more than three antibiotics, and three isolates were most resistant to 10 antibiotics. The results indicate that the serotypes of Salmo- nella isolated from retail fresh pork and beef in Nanning are complex, and the multi - drug resistance of the isolates is a serious problem.