藏茵陈上调大鼠肝细胞膜转运蛋白多耐药相关蛋白3和核受体孕烷X受体表达

Effect of Artemisiae capillaries on the expressions of hepatic membrane protein Mrp3 and nuclear receptor Pxr in rats

目的:观察藏茵陈对正常大鼠多耐药相关蛋白3(MRP3)和核受体孕烷X受体(PXR)表达的影响。方法:将10只SD大鼠随机分为藏茵陈组5只,藏茵陈100 mg·kg-1·d-1灌胃7 d;对照组5只,同体积0.9%氯化钠注射液灌胃。7 d后麻醉处死2组大鼠,取肝脏组织行HE染色检测肝脏形态变化,分别提取肝脏组织RNA、膜蛋白及核蛋白,采用实时聚合酶链式反应和蛋白免疫印迹检测膜转运蛋白MRP3和核受体PXR在转录与蛋白水平的表达变化。结果:给予藏茵陈后,与0.9%氯化钠注射液比较,未影响肝脏组织的形态结构;藏茵陈可显著刺激正常大鼠肝细胞膜转运蛋白MRP3表达(P<0.01);也可明显上调核受体PXR水平增高(P<0.01)。结论:藏茵陈刺激大鼠肝细胞膜蛋白MRP3的表达上调可能与核受体PXR途径相关。

Objective：To observe the effects of Artemisiae capillaris on the expressions of muhidrug resistance-associated protein 3 （MRP3） and nuclear pregnane X receptor （PXR） in normal rat liver. Methods：Ten male Sprague-Dawley （SD） rats were randomly divided into the Artemisiae capillaris group and control group （5 rats each group）. The Artemisiae capillaris group and control group were treated with gavage of Artemisiae capillaris（ 100 mg·kg^-1· d^-1） and gavage of 0.9% sodium chloride. Two groups were sacrificed after 7 days. The morphological change of liver tissue was observed using HE staining, the total RNA and membrane or nuclear protein were extracted from the liver tissue of two groups. The mRNA and protein expressions of MRP3 and PXR were detected by Real- time PCR and Western blotting, respectively. Results： Compared with the control group, the morphological structure of liver tissue in Artemisiae capillaris group was not influenced. Artemisiae capillaris could significantly up-regulate the protein expressions of MRP3 and PXR in rat liver（ P 〈 0.01 ）. Conclusions： Artemisiae capillaris can up-regulate the expression of MRP3 and PXR in rat liver.