破骨细胞对不同底物的吸收活性比较

Effect of osteoclasts on the absorption activity of different substrates

目的:比较破骨细胞对牛皮质骨片及牙本质片的吸收活性。方法:采用25 ng/ml巨噬细胞集落刺激因子+30 ng/ml核因子κB受体活化因子配体诱导RAW264.7细胞分化获取破骨细胞,并分别与牛皮质骨片及牙本质片共培养。通过检测抗酒石酸酸性磷酸酶(TRAP)染色阳性细胞数、骨吸收陷窝面积,比较破骨细胞在不同底物上的骨吸收活性。结果:与不同底物共培养形成的TRAP阳性细胞数差异无统计学意义(P>0.05),而牛皮质骨片吸收陷窝的面积显著大于牙本质片(P<0.01)。结论:破骨细胞对牛皮质骨片的吸收活性强于牙本质片。

Objective：To compare the effects of osteoclasts on the absorption activities of the bovine cortical bone slices and dentine discs. Methods：Osteoclasts were harvested from RAW264. 7 cells induced by 25 ng/ml M-CSF combined with 30 ng/ml RANKL, which was cocultured with bovine cortical bone slices and dentine discs,respectively. The number of positive cells and area of resorption lacunae were detected by antitartaric acid phosphatase staining, and the absorption activities of different substrates were compared. Results：The difference of the TRAP positive cells in different substrates coculture was not statistically significant（P＆gt;0. 05）. The area of resorption lacunae in bovine cortical bone slices was significantly more than that in dentine discs（P〈0. 01）. Conclusions：Compared with the dentine discs,the absorption activity of osteoclasts on bovine cortical bone slices is strong.