脂蛋白脂酶基因rs328多态性与汉族人脑梗死的相关性研究

Study on the correlation between rs328 polymorphism of lipoprotein lipase gene and Chinese Han cerebral infarction

目的探讨脂蛋白脂酶(LPL)基因rs328多态性与汉族人脑梗死的相关性。方法选择150例汉族脑梗死患者,按类肝素药物治疗急性缺血性脑卒中试验(TOAST)分型标准,分为大动脉粥样硬化(LAA)亚组和小动脉闭塞(SAO)亚组,并选择160名健康体检人群为对照组。以rs328位点为遗传标记,应用聚合酶链反应分析LPL基因rs328多态性与脑梗死的相关性。结果 1脑梗死组基因型频率与对照组相比,差异无统计学意义(P>0.05)。LAA亚组基因型频率与对照组相比,差异有统计学意义(P<0.05)。2LAA亚组rs328位点G等位基因频率为12.50%,与对照组的6.56%比较,LAA亚组明显增高[P<0.05,OR=2.034,95%CI(1.183,2.485)]。SAO亚组rs328位点等位基因频率与对照组差异无统计学意义(P>0.05)。结论 LPL rs328位点基因多态性与汉族人脑梗死的发病相关,G等位基因携带者患大动脉粥样硬化性脑梗死的风险高于非携带者。

Objective To study the correlation between rs328 polymorphism of lipoprotein lipase （LPL） gene and Chinese Han cerebral infarction. Methods One hundred and fifty Han patients with cerebral infarction were selected as the infarction group, and were divided into large-artery atherosclerosis （LAA） subgroup and small-artery occlusion （SAO）subgroup according to TOAST classification standard. One hundred and sixty healthy people were selected as the control group. The relationship between rs328 polymorphism of LPL gene and cerebral infarction were analyzed by polymerase chain reaction, with rs328 site as a genetic marker. Results ①There was no statistically significant difference of genotypic frequency between cerebral infarction group and con- trol group （P〉0.05）. There was statistically significant difference of genotypie frequency between LAA sub- group and control group （P〈0.05）. ②In rs328 site, the allele frequency was 12.50%in LAA group, higher than 6.56 % in control group[P〈0.05,OR= 2. 034,95 % CI （1. 183,2. 485）]. There was no statistical difference of allele frequency between SAO subgroup and control group in rs328 site（P〉0.05）. Conclusion The rs328 poly- morphism of LPL gene is related with Chinese Han cerebral infarction. Carriers of G allele have the higher risks of LAA cerebral infarction than non carriers.