常压室温等离子体诱变选育高产TTMP菌株

Breeding of a Bacillus subtilis Mutant Strain with High Yield of TTMP by Atmospheric Room Temperature Plasma

从新疆伊犁肖尔布拉克酒厂生产的酱香型高温大曲中筛选得1株能代谢四甲基吡嗪(TTMP)的枯草芽孢杆菌(Bacillus subtilis)R19,以该菌株为出发菌株,采用常压室温等离子体诱变系统(ARTP)进行诱变,根据脱脂奶粉平板初筛、摇瓶发酵复筛,连续传代培养后得到遗传稳定的TTMP产量较高的突变株T451,该菌株摇瓶发酵生产TTMP的产量为1.19 g/L,约为出发菌株产量的4.45倍。

In this study, a TTMP-producing Bacillus subtilis strain R19 was screened from Jiangxiang high-temperature Daqu in Xiaoerbulake Distillery in Xinjiang. Then R19 was used as the starting strain to obtain its mutant strains through atmospheric room temperature plasma （ARTP）. And a mutant strain T451 with high yield of TTMP and stable heredity was obtained through plat screening and flask fermentation. TT-MP yield of T451 in flask fermentation was as high as 1.19 g/L, 4.45 times of that of the starting strain.