Fractalkine对人外周血单个核细胞IL-8表达的影响及缬沙坦的干预作用

Effect of Fractalkine on IL-8 expression of monocytes in peripheral blood and the interventional effect of Valsartan

目的观察趋化因子Fractalkine(FKN)对人血单个核细胞IL-8表达的影响,蛋白激酶C(PKC)在其中的作用,以及缬沙坦的干预效应。方法利用密度梯度离心法分离人外周血单个核细胞(PBMC);将提取的单个核细胞分5组,分别为:空白对照组、FKN组、Ro31-8220(PKC阻断剂)组、FKN+Ro31-8220组、FKN+缬沙坦组;各组PBMC分别在加药培养12 h和24 h后,用酶联免疫吸附实验(ELISA)法检测各组细胞培养液中的IL-8表达。结果 (1)细胞培养12 h或24 h后,与空白对照组相比,Ro31-8220组IL-8表达无明显差异(P>0.05);(2)FKN组较空白对照组IL-8表达明显减少(P<0.05),FKN+Ro31-8220较FKN组IL-8表达明显增加(P<0.05),FKN+缬沙坦组较FKN组IL-8表达明显减少(P<0.05)。(3)细胞培养24 h后,各组IL-8表达均较12h明显减少(P<0.05)。结论 FKN通过PKC途径抑制IL-8的表达,缬沙坦可加强这一抑制作用。

Objective To observe the effect of Fractalkine （FKN） on IL -8 expression of monocytes in peripheral blood and the function of Valsartan during the process. Methods Monocytes from fresh peripheral blood of healthy volunteers were isolated by the density gradient centrifugation. The monocytes isolated were divided into five groups： control, FKN, Ro31 -8220（PKC inhibitor）, FKN ＋ Ro31 -8220, FKN ＋ Valsartan. The supernatants of monocytes were collected at 12 h and 24 h from each group. And the IL - 8 expressions of monocytes in every group were detected with ELISA. Results Compared with control group, IL -8 expressions decreased in FKN group （P 〈 0.05 ）, while not significantlyin Ro31 - 8220 group （P 〉0.05）. Compared with FKN group, IL- 8 expression increased in FKN ＋ Ro31 -8220 group （P 〈 0. 05 ）, while decreased in FKN ＋ Valsartan group （ P 〈 0. 05 ） . After 24 h, IL - 8 expression in each group was significantly decreased than 12 h before （P 〈 0. 05 ）. Conclusion FKN could inhibit IL - 8 expression in monocytes by PKC pathway. Valsartan may enhance the inhibition of FKN.