HPLC法测定双氢青蒿素哌喹片中双氢青蒿素的含量

Content Determination of Dihydroartemisinin in Dihydroartemisinin and Piperaquine Phosphate Tablets by HPLC

目的：建立测定双氢青蒿素哌喹片中双氢青蒿素含量的测定方法。方法 ：采用高效液相色谱法。色谱柱为Waters YMC-Pack ODS-AQ,流动相为乙腈-水（梯度洗脱）,流速为1.0 ml/min,检测波长为216 nm,柱温为30℃,进样量为20μl。结果：双氢青蒿素质量浓度在0.009 650~1.930 mg/ml范围内与峰面积呈良好线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD≤0.5%;平均加样回收率为100.76%,RSD为0.95%（n=9）。结论：该方法准确、简便、快速,可用于双氢青蒿素哌喹片中双氢青蒿素的含量测定。

OBJECTIVE： To establish a method for content determination of dihydroartemisinin in Dihydroartemisinin and piperaquine phosphate tablets. METHODS： HPLC was performed on the column of Waters YMC-Pack ODS-AQ with mobile phase of acetonitrile-water （gradient elution） at the flow rate of 1.0 ml/min, the detection wavelength was 216 nm,column temperature was 30 0（2, and injection volume was 20 μl. RESULTS：The linear range of dihydroartemisinin was 0.009 650-1.930 mg/ml（r=0.999 9） ; RSDs of precision, stability and reproducibility tests were no more than 0.5% ; average recovery was 100.76% （RSD=0.95%, n= 9）. CONCLUSIONS： The method is simple, rapid and accurate, and can be used for the content determination of dihydroartemisinin in Dihydroartemisinin and piperaquine phosphate tablets.