不同产地木瓜中绿原酸、原儿茶酸和总酚的含量测定

Content Determination of Chlorogenic Acid,Protocatechuic Acid and Total Phenolics in Chaenomeles sinersis from Different Areas

目的：建立木瓜中绿原酸、原儿茶酸和总酚的含量测定方法,比较不同产地木瓜总酚及两种酚酸的含量。方法：采用双波长切换高效液相色谱法测定绿原酸和原儿茶酸,色谱柱为Shim-pack CLC-ODS（M）,流动相为乙腈-0.1%磷酸水溶液（15∶85,V/V）,检测波长为259 nm（原儿茶酸）和325 nm（绿原酸）,切换时间为进样14 min后;以原儿茶酸为指标成分,以Folin-Ciocalteau比色法测定总酚含量。结果：两种定量分析方法的精密度、重复性、加样回收率和标准曲线均通过方法学验证。木瓜总酚质量分数为0.87%~3.77%,平均值2.16%;绿原酸质量分数为0.053%~0.387%,平均值0.192%;原儿茶酸质量分数为0.024%~0.541%,平均值0.087%;不同产地木瓜总酚含量高低顺序为：云南〉安徽宣城〉四川〉湖北;不同产地绿原酸和原儿茶酸总量高低顺序同总酚。不同产地木瓜所测成分含量存在差异,但总酚含量与绿原酸和原儿茶酸总量存在一定正相关性,Pearson相关系数为0.719（P〈0.01）。结论：所建方法简便、准确、重复性好,可用于木瓜中绿原酸、原儿茶酸和总酚的含量测定。

OBJECTIVE： To establish a method for the content determination of chlorogenic acid, protocatechuic acid and total phenolics in Chaenomeles sinensis, compare the content of total phenolics and 2 phenolic acids from different areas. METHODS： Wavelength switching HPLC method was conducted to determine the chlorogenic acid and protocatechuic acid. The column was Shim-pack CLC-ODS（M） with the mobile phase of acetonitrile -0.1% phoephoric acid （15：85, V/V）, the detection wavelength was 259 nm （for protocatechuic acid） and 325 nm （for chlorogenic acid） and the switching time was 14 min. With the index of protocatechuic acid, Folin-Ciocalteau colorimetric method was conducted to determine the total phenolics. RESULTS： The 2 methods of quantitative analysis showed that the precision, repeatability, recoveries and standard curves were all validated by methodology. The mass fraction of total phenolics was 0.87%-3.77% with the average of 2.16%; the chlorogenic acid was 0.053%-0.387% with the average of 0.192% and the protocatechuic acid was 0.024%-0.541% with the average of 0.087%. The order of total phenolics content in C. sinensis from different areas was Yunnan〉Anhui Xuancheng〉Sichuan〉Hubei and the order of total amount of chlorogenic acid and protocatechuic acid from different areas was the same as the total phenolics. There were differences among the contents of C. sinensis from different areas, however, the positive correlation was found between the content of total phenolics and the total amount of chlorogenic acid and protocatechuic acid with the pearson correlation coefficient of 0.719 （P〈0.01）.CONCLUSIONS ： The established method is simple, accurate and reproducible and can be used for the content determination of chlorogenic acid, protocatechuic acid and total phenolics in chaenomelis fructus.