5种不同种植密度蕲艾中桉油精的含量比较

Comparison of Content of Eucalyptol in Artemisia argyi of 5 Different Planting Densities

目的：比较5种不同种植密度蕲艾中桉油精的含量。方法：采用气相色谱法。色谱柱为HP-5毛细管色谱柱,以环己酮为内标,载气为氮气,流速为0.6 ml/min,进样口温度240℃,检测器温度250℃,分流比为10∶1,进样量为1μl。结果：桉油精质量浓度在0.420 4~1.261 2 mg/ml范围内与峰面积呈良好的线性关系（r=0.999 9）;精密度、稳定性、重复性试验的RSD≤0.92%;平均加样回收率为99.6%,RSD为1.4%（n=6）。结论：该方法操作简便、快速,结果准确、可靠,重复性良好,可用于蕲艾中桉油精含量的测定。建议蕲艾最佳种植密度为行距30 cm×40 cm。

OBJECTIVE： To compare the contents of eucalyptol in Artemisia argyi of 5 different planting densities. METH- ODS： Gas chromatography method was performed on the column of HP-5 capillary column, and cyclohexanone was selected as internal standard, the carrier gas was nitrogen at a flow rate of 0.6 ml/min, the volume temperature was 240 %, the detector temperature was 250 ℃, the split ratio was 10： 1, and the injection volume was 1 μl. RESULTS： The linear range of eucalyptol was 0.420 4-1.261 2 mg/ml（r=0.999 9）; RSDs of precision, stability and reproducibility tests were no more than 0.92%; average recovery was 99.6% （RSD= 1.4%, n=6）. CONCLUSIONS： The method is simple, rapid, accurate, reliable and reproducible, and can be used for the content determination of eucalyptol in A. argyi. The optimal planting density ofA. argyi was spaced 30 crux40 cm.