摘要
目的:建立测定甲基丙烯酸-丙烯酸乙酯共聚物中有关物质含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent C18,流动相为甲醇-磷酸盐缓冲液(30∶70,V/V),流速为1.0 ml/min,检测波长为202 nm,柱温为20℃,进样量为20μl。结果:甲基丙烯酸和丙烯酸乙酯质量分数在0.001%~0.015%范围内均与其峰面积呈良好的线性关系(r=0.999 4、0.999 1);精密度、稳定性和重复性试验的RSD均〈2%;甲基丙烯酸平均加样回收率为86.59%,RSD为2.3%(n=9),丙烯酸乙酯平均加样回收率为91.24%,RSD为3.5%(n=9)。结论:该方法简单、准确、重复性好,可用于甲基丙烯酸-丙烯酸乙酯共聚物中有关物质的含量测定。
OBJECTIVE: To establish a method for the determination of related substances in methylacrylic acid - ethyl acrylate copolymer. METHODS: HPLC was performed on the column of Agilent C18 with mobile phase of methanol-phosphate buffer (70: 30, V/V) at the flow rate of 1.0 ml/min, the detection wavelength was 202 nm, column temperature was 20 92, and the sample size was 20 μl. RESULTS: The linear range was 0.001%-0.015% for both methylacrylic acid (r=0.999 4) and ethyl acrylate (r= 0.999 1 ); RSDs of precision, stability and reproducibility tests were lower than 2%; the average recoveries of methylacrylic acid and ethyl acrylate were respectively 86.59% (RSD = 2.3 %, n= 9) and 91.24% (RSD= 3.5 %, n=9). CONCLUSIONS: The method is simple, accurate and reproducible, and can be used for the contents determination of related substances in methyl acrylic acid-ethyl acrylate copolymer.
出处
《中国药房》
CAS
北大核心
2015年第24期3436-3438,共3页
China Pharmacy