HPLC法测定甲基丙烯酸-丙烯酸乙酯共聚物中有关物质的含量

Determination of Related Substances in Methylacrylic Acid-Ethyl Acrylate Copolymer by HPLC

目的：建立测定甲基丙烯酸-丙烯酸乙酯共聚物中有关物质含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent C18,流动相为甲醇-磷酸盐缓冲液（30∶70,V/V）,流速为1.0 ml/min,检测波长为202 nm,柱温为20℃,进样量为20μl。结果：甲基丙烯酸和丙烯酸乙酯质量分数在0.001%~0.015%范围内均与其峰面积呈良好的线性关系（r=0.999 4、0.999 1）;精密度、稳定性和重复性试验的RSD均〈2%;甲基丙烯酸平均加样回收率为86.59%,RSD为2.3%（n=9）,丙烯酸乙酯平均加样回收率为91.24%,RSD为3.5%（n=9）。结论：该方法简单、准确、重复性好,可用于甲基丙烯酸-丙烯酸乙酯共聚物中有关物质的含量测定。

OBJECTIVE： To establish a method for the determination of related substances in methylacrylic acid - ethyl acrylate copolymer. METHODS： HPLC was performed on the column of Agilent C18 with mobile phase of methanol-phosphate buffer （70： 30, V/V） at the flow rate of 1.0 ml/min, the detection wavelength was 202 nm, column temperature was 20 92, and the sample size was 20 μl. RESULTS： The linear range was 0.001%-0.015% for both methylacrylic acid （r=0.999 4） and ethyl acrylate （r= 0.999 1 ）; RSDs of precision, stability and reproducibility tests were lower than 2%; the average recoveries of methylacrylic acid and ethyl acrylate were respectively 86.59% （RSD = 2.3 %, n= 9） and 91.24% （RSD= 3.5 %, n=9）. CONCLUSIONS： The method is simple, accurate and reproducible, and can be used for the contents determination of related substances in methyl acrylic acid-ethyl acrylate copolymer.