摘要
目的:考察暑热宁口服液中挥发油含测影响因素,为工艺制定、样品制备、质量标准制定、服药方式等提供依据。方法:以百秋李醇、麝香草酚-香荆芥酚为含测指标,考察过滤方式、提取方式、提取时间、正己烷量、计算方法、p H值、盐析、灭菌、药液、稀释等因素对含测值的影响。结果:过滤和调p H值后各含测指标均降低,提示工艺制定时不可过滤和调节p H值;先超声再提油含测值增大约5%,提示服药前应先温水加热混匀;样品制备宜用成品提油,加4 m L正己烷,提取2.5 h;内标法与外标法含测结果一致;灭菌跟药液本身可使百秋李醇减少约85%,麝香草酚-香荆芥酚减少约21%~24%。结论:诸多因素可以影响挥发油含测值,在进行工艺、质量标准制定和用药指导时要综合考虑。
Objective:It was to investigate the affecting measurement factors in the volatile oil containing of Shure'ning oral liq- uid, providing the basis for technology development, sample preparation, quality standard and the way of medieine. Methods:The patchouli alcohol and thymol - oxyzymol was the measurement indicators, we investigated the influences of filtering methods, ex- traction mode, extraction time, N - hexane quantity, ealeulation method, pH value, salting out, sterilization, liquid and dilution on measured values. Results:After filtration and adjusting pH value, the containing indexes decreased, prompting no filtering or adjus- ting pH value during the process. Ultrasonic before oil extraction made the measured values increase by about 5% ,prompting be- fore taking the medicine it should be heated by warmed water and mixed;sample preparation should use finished oil extraction, adding 4 mL n - hexane, extracting for 2. 5 h. Internal standard method and external standard method had the same measured re- sults consistently. Sterilization and liquid itself can make the patchouli alcohol reduce around 85%, thymol -oxyzymol decrease a- bout 21% -24%. Conclusion:Many factors can affect the volatile oil measured values. In the process, quality standards and guide- lines should be considered.
出处
《辽宁中医杂志》
CAS
北大核心
2015年第8期1492-1495,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
"重大新药创制"科技重大专项项目(2010ZX09102-201)
关键词
暑热宁口服液
百秋李醇
麝香草酚-香荆芥酚
含量测定
影响因素
Shure'ning Oral Liquid
patchouli alcohol
thymol - oxyzymol
content determination
influencing factor