葛花亚慢性毒性研究

A study on subchronic toxicity of Flos Puerariae

目的了解葛花亚慢性毒性,为更好地开发利用葛花提供科学依据。方法依据《食品安全性毒理学评价程序和方法》进行试验,选取80只清结级SD大鼠,按体质量分成4组,给予剂量分别为0、6.25、12.50、25.00g/kg BW（以葛花计）,每组20只,雌雄各半。受试组以葛花水及其醇提取物作为受试物,每天按10.0 ml/kg BW进行灌胃,连续90 d。第45天采尾血进行血常规及生化指标检测;第90天采血测定血常规及生化指标,取肝、肾、脾、睾丸称重,并对肝、肾、脾、胃肠、睾丸、卵巢作病理学检查。结果各受试组大鼠体质量、进食量、食物利用率、脏体比各项指标与对照组对应指标比较,差异无统计学意义（P〉0.05）;试验中期及结束时,个别受试组大鼠血常规、血生化指标与对照组对应指标比较,差异有统计学意义（P〈0.05）,但均在本实验室正常值范围内,没有生物学意义;组织病理学观察显示个别动物出现轻度的病理改变。结论葛花剂量在6.25~25.00 g/kg BW范围内对清洁级SD大鼠生长发育未见不良影响及毒副作用。

Objective The aim of this study was to investigate subchronic toxicity of Flos Puerariae. Methods According to procedures and methods for toxicological assessment of food （2003 edition） , eighty SD mice were randomly divide into four groups （ 10 male and 10 female in each group）. 0, 6.25, 12.5, 25.0 g/kg BW of Flos Puerariae extract were given by gavage for 90 days respectively. On the 45th day, samples of blood were collected from tails to perform complete blood count and biochemical tests. After 90 days, samples of blood were collected and were used for blood count and biochemical tests; livers, kidneys, spleens and testicles （ovaries） were weighed; pathological examinations of livers, kidneys, spleens stomach intestines testicles and ovaries were conducted. Results The results showed that indexes including weight, food intakes, food utilizations, and organ-body ratios in treatment groups are not significant different from those in the control group （P 〉 0. 05） ; in the interim and at the end of experiments, the blood count and biochemical tests of some certain treatment groups have statistical difference （P 〈 0. 05 ） ; however, the difference is in a normal range and has no biological significance; minor pathological changes have been discovered in a few treatment groups through the histopathology examination. Conclusion Flos Puerariae extracts have no adverse effect on the growth performance of mice under the doses of pueraria flower in a range of 6.25-25.00 g/kg BW. Moreover, Flos Puerariae extracts haven＇t caused obvious toxic and side effects on experimental animals.