大鼠骨髓间充质干细胞对雄性生殖系统的修复作用

Bone marrow mesenchymal stem cells to repair the reproductive system of male azoospermia rats

目的:探讨大鼠骨髓间充质干细胞(BMSCs)在无精子症大鼠模型中对睾丸内环境的修复能力。方法:将同种异体BMSCs移植入无精子症模型大鼠睾丸生精小管中,注射后30 d HE染色观察生精小管细胞组成和结构,免疫组化检测CD44、CD106和c-kit表达情况。结果:与正常大鼠相比,20 mg/kg白消安组中大鼠附睾中精子数量明显减少(P<0.01)。分离得到的BMSCs表达CD44和CD106,而不表达c-kit。BMSCs注射移植30 d后,HE染色显示移植组生精小管内形成新的细胞,部分细胞表达CD106,部分细胞表达生殖细胞表面标记c-kit。结论:BMSCs在移植组无精子症大鼠生精小管中能够分化为生殖细胞,对受损的不育大鼠生精小管进行修复。

Objective： To study the ability of bone marrow mesenchymal stem cells （BMSCs） to repair the internal environment of the testis in male azoospermia rats. Methods ： We established azoospermia models in 22 six-week-old male SD rats by intraperito- neal injection of busulfan at 20 mg per kg body weight. We transplanted allogeneic rat BMSCs （rBMSCs） into the testieular seminife- rous tubules of the model rats and, 30 days after transplantation, observed the composition and structure of the seminiferous tubular cells by HE staining and detected the expressions of CD44, CD106, and c-kit in the rBMSCs by immunohistoehemistry. Results ： The number of epididymal sperm was significantly reduced in the model rats as compared with the normal controls （P 〈 0.01 ）. CD44 and CD106, but not c-kit, were expressed in the isolated rBMSCs. At 30 days after transplantation of rBMSCs, lots of new cells were ob- served in the seminiferous tubules, some expressing CD106 and some expressing the germ cell surface marker c-kit. Conclusion： BMSCs can transdifferentiate into germ cells and repair the damaged seminiferous tubules of sterile rats.